新收获玉米生霉粒霉斑颜色与优势菌株分析研究

Study and Analysis on the Infection of Different Moldy Spot Color Grains of Newly Harvested Maize

玉米作为全球重要的粮食作物和饲料来源,在其种植、收获与储存过程中容易受到真菌污染,尤其是真菌毒素的污染,对人类健康和食品安全构成重大威胁。本研究通过收集辽宁不同地区的新收获玉米样本,采用微生物学培养、形态学观察、分子生物学鉴定及高通量测序分析等相结合的方法确定不同霉斑颜色(粉色、白色、绿色和黑色)籽粒的附生菌和内生菌带菌情况,探讨霉斑颜色与特定真菌种群之间的关联性,以期通过外观特征初步判断籽粒的潜在食品安全风险,为玉米的安全储存和真菌毒素污染的预防提供科学依据。

As an important food crop and feed source in the world,maize is vulnerable to fungal contamination during its cultivation,harvesting and storage,especially mycotoxin contamination,which poses a major threat to human health and food safety.In this study,the newly harvested maize samples from diferent regions of Liaoning Province were collected,and the methods of microbiological culture,morphological observation,molecular biological identification and high-throughput sequencing analysis were used to determine the epiphytic and endophytic bacteria in the grains with different color spots(pink,white,green and black).The correlation between the color of the moldy spots and the specific fungal population was discussed in order to preliminarily judge the potential health risks of the grains through the appearance characteristics,and provide a scientific basis for the safe storage of maize and the prevention of mycotoxin contamination.