贵州省安顺烟区烟草主要病毒种类及复合侵染类型分析

Identification and analysis of main viral species and mixed infection types of tobacco in Anshun tobacco growing area of Guizhou Province

为明确贵州省安顺烟区烟草主要病毒毒源种类和侵染类型,2023年从安顺烟区采集表现为花叶、叶脉扭曲坏死、畸形、闪电斑和斑点黄化等症状的烟草样品306份,并利用烟草花叶病毒(TMV)、黄瓜花叶病毒(CMV)、马铃薯Y病毒(PVY)、烟草脉带花叶病毒(TVBMV)和辣椒脉斑驳病毒(ChiVMV)5种主要病毒病特异性引物进行RT-PCR扩增检测,统计病毒的检出率并分析复合侵染类型。结果显示:总病毒检出率为89.87%。其中,TMV占比最高,为81.70%;ChiVMV次之,为67.97%;CMV占比最低,为16.34%。复合侵染是主要的侵染类型,检出率为68.30%。其中,ChiVMV+TMV类型占比最高,为22.88%,其次是ChiVMV+TMV+TVBMV、ChiVMV+TMV+PVY和ChiVMV+TMV+CMV 3种类型,分别占11.11%、10.46%和9.15%。贵州省安顺烟区烟草病毒病的优势毒源为TMV和ChiVMV,复合侵染普遍发生,以ChiVMV+TMV复合侵染类型为主。

To clarify the main species and infection types of tobacco viral diseases in Anshun tobacco growing areas of Guizhou Province,306 diseased samples with symptoms such as mosaic,necrotic veins,abnormal shapes,lightning spots,and yellow spots were collected from Anshun tobacco growing areas in 2023.RT-PCR was performed using specific primers for 5 major viral diseases of tobacco mosaic virus(TMV),cucumber mosaic virus(CMV),potato Y virus(PVY),tobacco vein banding mosaic virus(TVBMV)and chilli veinal mottle virus(ChiVMV),and the detection rates of the viruses were calculated and the mixed infection types were analyzed.The results showed that the total detection rate of the viral diseases was 89.87%,and among all the diseases,TMV(81.70%)ranked the highest,followed by ChiVMV(67.97%),and CMV ranked the lowest(16.34%).The main type of infection was mixed infection with a detection rate of 68.30%.Among them,ChiVMV+TMV was the main type with a detection rate of 22.88%,followed by ChiVMV+TMV+TVBMV,ChiVMV+TMV+PVY,and ChiVMV+TMV+CMV with detection rates of 11.11%,10.46%,and 9.15%,respectively.TMV and ChiVMV were the dominant viral species of the tobacco viral diseases from the area,mixed infection occurred frequently and ChiVMV+TMV was the main mixed type.