甘肃不同产地党参性状、无机元素、党参炔苷差异及相关性研究

Study on Difference and Correlation of Traits,Inorganic Elements and Lobetyoli of Dangshen(Codonopsis Radix)from Different Origins in Gansu Province

目的:研究甘肃不同产地党参性状、无机元素及党参炔苷(LBT)差异,分析彼此间的相关性,为党参质量评价及产地归属提供依据。方法:收集甘肃9个产地党参药材,进行性状比较,并采用原子吸收光谱法测定党参中10种无机元素含量;采用高效液相色谱法(HPLC)测定LBT含量;并统计分析无机元素和LBT含量,联合性状进行相关性研究。结果:不同产地党参性状、无机元素及LBT含量存在差异,各元素平均含量高低顺序大致为K>Mg>Ca>Na>Fe>Mn>Co>Zn>Ni>Cu;LBT含量范围为0.75~6.50 mg/g。聚类分析将其分为四类。相关性分析表明不同产地党参中LBT含量与Mn、Na、Mg含量呈显著正相关;Co含量与党参“菊花心”特征呈负相关;Fe、Ca含量与党参根头部横环纹特征存在正相关。正交偏最小二乘法判别分析发现LBT、K、Mn、Ca、Na、Mg是鉴别不同产地党参的主要成分元素。结论:不同产地党参性状、无机元素及LBT含量表现出一定的相关性,三方面联合可有效评价党参质量及产地归属。

Objective:To study the differences of the traits,inorganic elements and lobetyoli(LBT)of Dangshen(Codonopsis Radix)in different origins of Gansu Province,and analyze the correlation to provide a reference for the quality evaluation of Dangshen and the attribution of origin.Methods:The samples of Dangshen from 9 origins were collected.The contents of 10 inorganic elements in Dangshen were detected using flame atomic absorption spectrometry.The content of LBT was determined by high performance liquid chromatography(HPLC).The contents of inorganic elements and LBT were analyzed statistically,and the correlation with the traits was studied.Results:There were differences in the traits,contents of inorganic elements and LBT of Dangshen fromdifferent origins. The average content of each element was mostly in the order of K>Mg>Ca>Na>Fe>Mn>Co>Zn>Ni>Cu. The LBT content ranged from 0.75 to 6.50 mg/g. Cluster analysis was performed to categorizethem into four groups. Correlation analysis showed that LBT content of Dangshen from different origins hadsignificant positive correlation with contents of Mn,Na and Mg. The content of Co had negatively correlationwith the trait of “radial striations” in Dangshen. The contents of Fe,Ca had positive correlation with thetrait of transverse circular striations in Dangshen. Orthogonal partial least squares discriminant analysisrevealed that LBT,K,Mn,Ca,Na and Mg were the main constituent elements for identifying Dangshen fromdifferent origins. Conclusion:The traits,contents of inorganic elements and LBT of Dangshen from differentorigins showed certain correlation,and these three aspects can be combined to effectively evaluate the qualityand origin attribution.