绿茶外泌体样纳米颗粒(ELNs)延缓人体成纤维细胞衰老作用研究

Study on the Delaying Effect of Green Tea Exosome Like Nanoparticles(ELNs)on the Aging of Human Fibroblasts

探究绿茶外泌体样纳米颗粒(ELNs)及其复方配伍对人体表皮成纤维细胞衰老模型的干预作用。通过超速离心法结合蔗糖密度梯度离心,提取纯化绿茶ELNs,采用透射电镜、粒径检测以及脂质组学分析对ELNs进行体外表征。CCK-8法证明24 h内ELNs对人体表皮成纤维细胞具有明显诱导增殖功能;生化分析和分子检测显示,ELNs能显著提高人体表皮成纤维细胞超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-px)活力,降低丙二醛(MDA)含量,并提高羟脯氨酸(HYP)、Ⅲ型胶原蛋白(COL3)含量,同时抑制基质金属蛋白酶-1(MMP-1)、P53和P16基因的表达。与番茄红素复配,具有显著协同抗衰老作用。结果表明绿茶ELNs及其复方能显著延缓人成纤维细胞衰老,主要干预细胞生化代谢过程和相关基因表达。

Exploring the intervention effect of green tea exosome like nanoparticles(ELNs)and their combination on human fibroblast aging model.Green tea ELNs were extracted and purified by ultracentrifugation combined with sucrose density gradient centrifugation.The ELNs were characterized in vitro using transmission electron microscopy,particle size detection,and lipidomics analysis.The CCK-8 method demonstrated that ELNs had a significant proliferative effect on human fibroblasts within 24 hours.Biochemical analysis and molecular detection showed that ELNs could significantly increase the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH px)in fibroblasts,reduce the content of malondialdehyde(MDA),and increase the content of hydroxyproline(HYP)and type III collagen(COL3),while inhibit the expression of matrix metalloproteinase-1(MMP-1),P53 and P16 genes.Combined with lycopene,it has a significant synergistic anti-aging effect.The results indicate that green tea ELNs and their compounds can significantly delay the aging of human fibroblasts,mainly intervening in cellular biochemical metabolism processes and related genes expression.