壳聚糖固定化石油烃降解菌的降解性能研究

Study on the Degradability of Hydrocarbon-degrading Bacteria Immobilized by Chitosan

本研究以筛选获得的一株石油烃降解菌HYHG-06为研究对象,选择壳聚糖为固定化载体材料,戊二醛为交联剂,通过交联反应固定化制备壳聚糖固定化石油烃降解菌;通过改变降解环境的温度、初始pH、盐度及石油烃浓度,探究4个因素对石油烃降解菌降解性能的影响。结果表明,固定化菌的降解性能优于游离菌,在温度30℃、初始pH8.0、盐度3.5%、石油烃浓度1.5%的环境条件下,固定化菌的石油烃降解率高达86.62%,可开发高效消油产品,为海上石油污染提供了一种高效可行的生化处理方法。

A strain of petroleum hydrocarbon degrading bacteria HYHG-06 was selected as the research object in this study.Chitosan as immobilized carrier material and glutaraldehyde as crosslinking agent was selected to prepare chitosan immobilized petroleum hydrocarbon degrading bacteria by crosslinking reaction.The effects of four factors on the degradability of immobilized bacteria were explored by changing the temperature,initial pH value,salinity and petroleum hydrocarbon concentration of the degradation environment.The results showed that the degradability of immobilized bacteria was better than that of free bacteria.Under the conditions of 30℃temperature,pH 8.0,salinity 3.5%and petroleum hydrocarbon concentration 1.5%,the petroleum hydrocarbon degradation rate of immobilized bacteria was as high as 86.62%.This can develop high efficient oil elimination products and provide an efficient and feasible biochemical treatment method for offshore oil pollution.