HS-SPME-GC-MS分析落地梅和狭叶落地梅不同部位挥发性成分

Analysis of Volatile Compositions in Different Parts of Lysimachia paridiformis Franch.and Lysimachia paridiformis var.stenophylla Franch.by HS-SPME-GC-MS

本研究基于顶空固相微萃取气质联用技术(HS-SPME-GC-MS)对落地梅和狭叶落地梅的根、茎和叶中的挥发性成分进行分析,通过NIST谱库检索和对照品比对等方式对其挥发性成分进行鉴定,并采用峰面积归一化法分析各成分的相对含量。结果显示,从落地梅的根、茎和叶中分别鉴定出29、41和42种挥发性成分,分别占总挥发性成分的93.63%、89.95%和88.30%,从狭叶落地梅的根、茎和叶中分别鉴定出44、40和41种挥发性成分,分别占总挥发性成分的91.33%、84.74%和84.84%,其中共检出组分74种,共有组分13种,二者的主要挥发性成分为倍半萜类和醛类。该实验为落地梅和狭叶落地梅不同部位挥发性成分的精准评价和高效利用提供参考依据。

Based on headspace solid-phase micro-extraction gas chromatography mass spectrometry(HS-SPME-GC-MS),the volatile compositions of the roots,stems and leaves of Lysimachia paridiformis Franch.and Lysimachia paridiformis var.stenophylla Franch.were studied.The volatile compositions were identified by the NIST database and by comparing with standard samples.The relative contents of each component were analyzed by the method of peak area normalization.In this work,29,41,and 42 volatile compounds were identified from the roots,stems,and leaves of Lysimachia paridiformis Franch.,accounting for 93.63%,89.95%,and 88.30%of the total volatile components,respectively.While,44,40,and 41 volatile compounds were identified from the roots,stems,and leaves of Lysimachia paridiformis var.stenophylla Franch.,accounting for 91.33%,84.74%,and 84.84%of the total volatile components,respectively.Totally,74 compounds were identified,with 13 shared in each part of materials.Sesquiterpenoids and aldehydes were the main volatile components in the two plants.This experiment can provide reference for accurate evaluation and efficient utilization of volatile components in different parts of Lysimachia paridiformis Franch.and Lysimachia paridiformis var.stenophylla Franch.