HPLC法同时测定风热感冒颗粒中5种成分

Simultaneous Determination of Five Components in Fengre Ganmao Granules by HPLC

目的:建立能同时测定风热感冒颗粒中5种成分含量的方法。方法:采用Agilent Eclipse Plus C18色谱柱(250 mm×4.6 mm,5μm),柱温25℃;1.0 mL/min乙腈-0.1%磷酸溶液梯度洗脱;多波长转换法测定,检测波长327 nm、278 nm;对绿原酸、牛蒡苷、异绿原酸A、连翘苷、芸香苷的含量测定方法进行方法学验证,将实验结果运用聚类分析(CA)进行评价。结果:在其各自相对应的浓度范围内,风热感冒颗粒中的5种成分均呈现出良好的线性关系(n≥0.9991),平均加样回收率分别为98.67%、97.44%、97.94%、98.44%、100.03%,RSD分别为1.61%、1.38%、1.70%、1.55%、0.96%;10批次样品中绿原酸、芸香苷、异绿原酸A、连翘苷、牛蒡苷成分含量分别为0.091 mg/g~0.227 mg/g、0.094 mg/g~0.258 mg/g、0.084 mg/g~0.274 mg/g、0.080 mg/g~0.217 mg/g、0.809 mg/g~2.584 mg/g;10批样品明显聚为两类,聚类情况为:s3、s10、s4、s2、s5、s9为一类,s1、s8、s6、s7为一类。结论:所建立的方法科学准确,可用于风热感冒颗粒的含量测定。

Objective:Establish a method for simultaneously determining the content of 5 components in Fengre Ganmao granules.s.Methods:Agilent Eclipse Plus C18 chromatographic column(250 mm×4.6 mm,5μm)Set the column temperature to 25℃;Gradient elution with acetonitrile 0.1%phosphoric acid solution as mobile phase at a flow rate of 1.0 mL/min;Multi wavelength conversion measurement,detection wavelength:327 nm,278 nm;Conduct methodological validation on the content determination methods of chlorogenic acid,burdock glycoside,isochlorogenic acid A,forsythrin,and rutin,Evaluate the experimental results using cluster analysis(CA).Results:The five components in Fengre Ganmao Granules showed good linear relationships within their respective concentration ranges(n≥0.9991),with average sample recovery rates of 98.67%,97.44%,97.94%,98.44%,100.03%,and RSD of 1.61%,1.38%,1.70%,1.55%,and 0.96%,respectively;The contents of chlorogenic acid,rutin,isochlorogenic acid A,forsythrin,and arctiin in 10 batches of samples were 0.091 mg/g~0.227 mg/g,0.094 mg/g~0.258 mg/g,0.084 mg/g~0.274 mg/g,0.080 mg/g~0.217 mg/g,and 0.809 mg/g~2.584 mg/g;10 batches of samples can be clustered into two categories,s3,s10,s4,s2,s5,and s9 can be clustered into one category,and s1,s8,s6,and s7 can be clustered into one category.Conclusion:The established method is scientific and accurate,and can be used for the content determination of Fengre Ganmao granules.