柠檬草乙醇提取物的化学成分研究

Chemical Components of Ethanol Extract of Cymbopogon citratus

通过重结晶、硅胶柱层析、反相柱色谱、制备薄层色谱、Sephadex LH-20凝胶柱色谱、高效液相色谱等方法对柠檬草乙醇提取物的化学成分进行了分离纯化,并通过^(1)HNMR、^(13)CNMR对其结构进行了鉴定。结果表明,从柠檬草乙醇提取物中分离得到8个化合物,分别鉴定为β-谷甾醇(1)、齐墩果酸(2)、乌苏醇(3)、山奈酚(4)、白桦脂酸(5)、坡塻酸(6)、4-O-β-D-吡喃葡萄糖基反式肉桂酸(7)、4-O-β-D-吡喃葡萄糖基顺式肉桂酸(8)。

We separated and purified the chemical components of ethanol extract of Cymbopogon citratus by recrystallization,silica gel column chromatography,reverse phase column chromatography,preparative thin-layer chromatography,Sephadex LH-20 gel column chromatography,and high performance liquid chromatography,and identified their structures by^(1)HNMR and^(13)CNMR.The results show that eight compounds are isolated from the ethanol extract of Cymbopogon citratus,which are identified asβ-sitosterol(1),oleanolic acid(2),uvaol(3),kaempferol(4),betalinic acid(5),pomolic acid(6),trans-p-coumaric acid 4-O-β-D-glucopyridineanoside(7),cis-p-coumaric acid 4-O-β-D-glucopyridineanoside(8).