砷对不同组织来源细胞株毒性效应相关长链非编码RNA表达的影响

Effect of arsenic on expression of long chain non-coding RNA associated to cell line toxic effects

目的探讨砷对乳腺、肝脏、肺细胞系长链非编码RNA(LncRNA)GAS5、HIF1A-AS1、PCAT6、FENDRR、SPRY4-IT1、DANCR、CCAT2、CCAT1、NEAT1、UCA1、HEIH、FALEC、ABALON表达的影响及其细胞特异性。方法将处于对数生长期的人乳腺癌(MDA-MB-231)细胞系、人肝癌(HepG2)细胞系、人肺腺癌(A549)细胞系分别暴露于不同浓度的亚砷酸钠(NaAsO2)培养72 h,检测LncRNA GAS5、HIF1A-AS1、PCAT6、FENDRR、SPRY4-IT1、DANCR、CCAT2、CCAT1、NEAT1、UCA1、HEIH、FALEC、ABALON表达水平。结果NaAsO2能够促进LncRNA GAS5、HIF1A-AS1、PCAT6、FENDRR、DANCR、CCAT2、CCAT1、NEAT1、UCA1、FALEC表达,抑制LncRNA SPRY4-IT1在MDA-MB-231细胞中的表达;促进LncRNA GAS5、PCAT6、FENDRR、SPRY4-IT1、CCAT2、NEAT1、UCA1、FALEC、ABALON表达,抑制LncRNA HIF1A-AS1、DANCR、HEIH在HepG2细胞中的表达;促进LncRNA SPRY4-IT1、DANCR、NEAT1、FALEC、ABALON表达,抑制LncRNA GAS5、HIF1A-AS1、CCAT1、UCA1、HEIH在A549细胞中的表达。结论砷可影响MDA-MB-231、HepG2、A549细胞中LncRNA GAS5、HIF1A-AS1、PCAT6、FENDRR、SPRY4-IT1、DANCR、CCAT2、CCAT1、NEAT1、UCA1、HEIH、FALEC、ABALON的表达,且具有细胞特异性。

Objective To explore the effects of arsenic on the expression of LncRNA GAS5,HIF1A-AS1,PCAT6,FENDRR,SPRY4-IT1,DANCR,CCAT2,CCAT1,NEAT1,UCA1,HEIH,FALEC,ABALON,and its cell specificity.Methods Human breast cancer(MDA-MB-231)cell line,human liver cancer(HepG2)cell line,and human lung cancer(A549)cell line in logarithmic growth phase were cultured with different concentrations of sodium arsenite(NaAsO2)for 72 h.Then,detect the expression levels of LncRNA GAS5,HIF1A-AS1,PCAT6,FENDRR,SPRY4-IT1,DANCR,CCAT2,CCAT1,NEAT1,UCA1,HEIH,FALEC,and ABALON.Results The results showed that NaAsO2 could promote the expression of LncRNA GAS5,HIF1A-AS1,PCAT6,FENDRR,DANCR,CCAT2,CCAT1,NEAT1,UCA1,FALEC,inhibit the expression of LncRNA SPRY4-IT1 in MDA-MB-231 cells;promote the expression of LncRNA GAS5,PCAT6,FENDRR,SPRY4-IT1,CCAT2,NEAT1,UCA1,FALEC,ABALON,inhibit the expression of LncRNA HIF1A-AS1,DANCR,HEIH in HepG2 cells;promote the expression of LncRNA SPRY4-IT1,DANCR,NEAT1,FALEC,ABALON and inhibit the expression of LncRNA GAS5,HIF1A-AS1,CCAT1,UCA1,HEIH in A549 cells.Conclusion The results suggested that arsenic could affect the expression of LncRNA GAS5,HIF1A-AS1,PCAT6,FENDRR,SPRY4-IT1,DANCR,CCAT2,CCAT1,NEAT1,UCA1,HEIH,FALEC,ABALON in MDA-MB-231,HepG2,and A549 cells,and which was cell-specific.