几何形态计量技术对热带臭虫的数字化标记及分析

Digital tag profiling of Cimex hemipterus by geometrical morphometry

目的研究热带臭虫形态结构特征信息,探究其特有的空间几何拓扑结构。方法使用超景深三维显微系统获取热带臭虫高清形态信息,利用几何形态计量学地标点技术确定热带臭虫形态变量框架,Adobe Illustrator软件描绘热带臭虫形态轮廓,TpsDig v2.32软件确定地标点并提取几何形态数据变量信息,TpsSuper v2.05软件对地标点进行普氏叠加分析。结果在海南省采集48只热带臭虫,包括11只若虫、22只雌虫及15只雄虫。于若虫背面构建49个地标点,腹面构建80个地标点;于雌虫背面构建48个地标点,腹面构建81个地标点;于雄虫背面构建51个地标点,腹面构建83个地标点。根据热带臭虫不同虫期的地标点结合其外部结构,共确立110个形态变量。普氏叠加将特征地标点映射出若虫、雌虫、雄虫的背面及腹面共计6个标准化模型,以表征热带臭虫不同虫期的归一化轮廓,建立了可视化几何模型。结论基于地标点编码规则的几何形态计量技术可作为热带臭虫的分类鉴别工具,并为不同虫期的热带臭虫提供相应的几何形态计量指标。

Objective To study the information on the morphological and structural characteristics,and to explore the unique spatial geometric topology of Cimex hemipterus.Methods The high-definition morphological information of Cimex hemipterus was obtained using an ultra-depth of field three-dimensional microscopy system.The morphological variable framework was determined for Cimex hemipterus using the landmark-based geometric morphometric landmark technique.The morphological contour of Cimex hemipterus was depicted in Adobe Illustrator software.The landmarks were determined and the variable information on geometric morphological data was extracted by TpsDig v2.32 software.The landmarks were characterized by procrustes superposition in TpsSuper v2.05 software.Results A total of 48 Cimex hemipterus samples were collected in Hainan province,including 11 nymphs,22 female adults and 15 male adults.A total of 49 landmarks were constructed on the dorsal surface and 80 on the ventral surface of nymph.A total of 48 landmarks were constructed on the dorsal surface and 81 on the ventral surface of female.A total of 51 landmarks were constructed on the dorsal surface and 83 on the ventral surface of male.A total of 110 morphological variables were established by combining the landmarks of different stages of Cimex hemipterus with their external structures.Procrustes superposition mapped characteristic landmarks to a total of 6 normalized models of the dorsal and ventral surfaces of nymph,female,and male to characterize the normalized profiles for a visual geometric model of different stages of Cimex hemipterus.Conclusion Geometrical morphometry based on landmark coding rules were used as a taxonomic identification tool for Cimex hemipterus,provided different geometrical morphometric index for different stages of Cimex hemipterus.