超高效液相色谱-三重四极杆串联质谱法检测凉茶中的碱性嫩黄O

Determination of Auramine O in Herbal Tea by Ultra Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry

建立了超高效液相色谱-三重四极杆串联质谱法(Ultra Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry,UPLC-MS/MS)测定凉茶中碱性嫩黄O的快速分析方法。以乙腈作为提取剂,样品经过涡旋混合、超声提取12 min和高速离心后,所得上清液用微孔滤膜过滤,采用UPLCMS/MS进行测定,以外标法定量。在质量浓度为0.10~20.00 ng·mL^(-1)时,碱性嫩黄O具有良好的线性关系,相关系数为0.9998,方法检出限为0.5μg·kg^(-1),定量限为1.0μg·kg^(-1)。添加水平为2.0~25.0μg·kg^(-1)时,样品加标回收率为86.7%~95.5%,相对标准偏差为3.5%~3.9%。该方法精密度好,准确度高,方便快捷,适用于凉茶中碱性嫩黄O含量的测定。

A rapid analytical method for the determination of auramine O in herbal tea was developed using ultra-performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-MS/MS).Acetonitrile was used as the extraction solvent,and the samples were vortex-mixed,subjected to ultrasonic extraction for 12 minutes,and then centrifuged at high speed.The supernatant was filtered through a membrane and analyzed using UPLCMS/MS with external standard quantification.Auramine O exhibited a good linear relationship in the concentration was 0.10 to 20.00 ng·mL^(-1),with a correlation coefficient was 0.9998.The method’s limit of detection was 0.5μg·kg^(-1),and the limit of quantification was 1.0μg·kg^(-1).The recovery rate at spiking levels ranging from 2.0μg·kg^(-1)to 25.0μg·kg^(-1)was between 86.7%and 95.5%,with the relative standard deviation was 3.5%to 3.9%.This method demonstrates high precision,accuracy,and efficiency,making it suitable for the quantification of auramine O in herbal tea.