MiRNA-21-5p靶向调节SATB1参与妊娠滋养层细胞的增殖和迁移作用的研究

MiRNA-21-5p participates in the proliferation and migration of gestational trophoblast cells via targeting and regulating SATB1

目的探究miRNA-21-5p对妊娠滋养层细胞增殖和迁移的作用及机制。方法RT-qPCR检测mi RNA-21-5p和核基质结合区结合蛋白1(special AT-rich binding protein 1,SATB1)在子痫前期(preeclampsia,PE)患者和正常孕妇的胎盘组织中的表达。CCK-8和EDU实验检测滋养细胞活力和增殖;Transwell检测细胞迁移;RT-qPCR和western blot检测MMP2、MMP9表达;Targetscan数据库和双荧光素酶实验预测并验证miRNA-21-5p与SATB1的结合。结果与对照组比较,PE患者胎盘组织中miRNA-21-5p表达显著增加,SATB1表达显著下降(P<0.05)。干扰miRNA-21-5p可增加细胞活力和增殖(P<0.001);增加细胞迁移和MMP2、MMP9表达(P<0.001)。MiRNA-21-5p靶向SATB1并负调控其表达(P<0.001)。干扰SATB1降低了干扰miRNA-21-5p对细胞增殖和迁移的促进作用(P<0.05)。结论miRNA-21-5p通过靶向上调SATB1促进妊娠滋养层细胞增殖和迁移,从而参与PE的发生发展。

Objective To investigate the effect and mechanism of miRNA-21-5p on the proliferation and migration of gestational trophoblast cells.Methods MiRNA-21-5p and Special AT-rich binding protein 1(SATB1)expressions in the placental tissues of preeclampsia(PE)patients and normal pregnant women were detected by RT-qPCR.Cell viability and proliferation were detected by CCK-8 and EDU assays.Cell migration was detected by transwell assays.MMP2,MMP9 and SATB1 expressions were detected by RT-qPCR and western blot.The binding of miRNA-21-5p to SATB1 was predicted and verified by Targetscan database and dual luciferase reporter assay.Results Compared with the control group,miRNA-21-5p expression in placental tissues of PE patients was significantly increased,and SATB1 expression was significantly decreased(P<0.05).Cell viability and proliferation were increased after miRNA-21-5p interference(P<0.001),cell migration and MMP2 and MMP9 expressions were increased(P<0.001).MiRNA-21-5p could bind to SATB1 and negatively modulated SATB1 expression(P<0.001).SATB1 interference reduced the effects of miRNA-21-5p knockdown on promoting cell proliferation and migration(P<0.05).Conclusion MiRNA-21-5p interference promoted gestational trophoblast cell proliferation and migration by targeting and up-regulating SATB1.