罗丹明6G酰肼上/下转换发光性能与次氯酸根的检测

The up/down conversion luminescence performance and hypochlorite detection of rhodamine 6G hydrazide

以罗丹明6G衍生物--罗丹明6G酰肼(R6GH)为分子探针,研究了其在甲醇/水混合溶剂中的上/下转换发光性能及其在次氯酸根检测方面的应用。除了传统的524 nm激发下的荧光发射之外,还发现这一分子探针体系在650 nm激发下会呈现出峰值在728 nm的荧光发射,以及在835 nm脉冲激光激发下在554 nm处的双光子上转换发射峰,这三种发射模式下的发光信号都会对次氯酸根产生响应,随着次氯酸根浓度的增大,发射强度随之增大,且呈良好的线性关系。从检测效果上看,下转换检测模式精度更高,但上转换检测模式的浓度响应范围更大。这种多波长激发下的上/下转换多模式检测探针,无需外加其他组分即可实现,能够灵活调整激发波长以满足不同应用场景的要求,为复杂环境下检测次氯酸根浓度提供了一种新的思路。

The molecular probe of rhodamine 6G hydrazide(R6GH),a derivative of rhodamine 6G,was used to investigate its up/down conversion luminescence in methanol/water mixed solvent and its application in hypochlo-rite detection.In addition to the traditional fluorescence emission under the 524 nm excitation,we found that this molecular probe exhibited fluorescence emission at 728 nm under 650 nm excitation.Furthermore,under pulsed laser excitation at 835 nm,a two-photon absorption upconversion emission at 554 nm was observed,All these three emission modes responded to hypochlorite ions,with increasing emission intensity and showing a good linear relationship with the concentration of hypochlorite ions.As for the detection performance,the downconversion de-tection mode had higher sensitivity,while the upconversion detection mode had a wider range of concentration response.This multi-mode detection probe based on up/down conversion luminescence under multi-wavelength excitation can be achieved without additional components,allowing for flexible adjustment of excitation wave-lengths to meet the requirements of different application occasions,providing a new approach for hypochlorite de-tection in complex environments.