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LINC00894在肺腺癌中的表达及其对肺腺癌细胞生物学功能的影响

Expression of LINC00894 in lung adenocarcinoma and its influence on the biological behavior of lung adenocarcinoma cells
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摘要 目的:研究长链非编码RNA(LINC00894)在肺腺癌中的表达水平与患者临床病理特征之间的关系,以及过表达LINC00894对肺腺癌细胞生物学功能的影响。方法:利用GEPIA2数据库,我们检测了LINC00894在肺腺癌组织中的表达情况及其与患者预后之间的关系,并利用TCGA数据库分析了LINC00894与患者临床病理特征关联。采用实时荧光定量PCR(RT-qPCR)检测人支气管上皮细胞系HBE、肺腺癌细胞系H1299及20例肺腺癌患者组织标本中LINC00894的表达;体外培养H1299细胞,实验分为转染空载体pEGFP-C1的Vector组和转染过表达载体pEGFP-C1-LINC00894的LINC00894组。CCK-8实验用于检测细胞增殖能力,划痕实验和Transwell小室实验用于评估细胞的迁移和侵袭能力,流式细胞术用于检测细胞凋亡。结果:LINC00894在肺腺癌组织和细胞中显示低表达,并与肿瘤的T分期和主要治疗结局[疾病进展(PD)、疾病稳定(SD)、部分缓解(PR)、完全缓解(CR)]显著相关。在肺腺癌中T分期越晚、治疗结局越不良,LINC00894的表达量越低。体外实验发现,当过表达LINC00894之后,细胞的增殖、迁移以及侵袭能力明显减弱,而凋亡能力增强。结论:上调LINC00894的表达水平可以抑制肺腺癌细胞的增殖、侵袭、迁移,同时促进细胞的凋亡。这表明LINC00894与肺腺癌的发生和发展密切相关。 Objective:To analyze the relationship between the expression level of long non-coding RNA(LINC00894)in lung adenocarcinoma and the clinicopathological features of patients,and to investigate the effect of overexpression of LINC00894 on the biological capabilities of lung adenocarcinoma cells.Methods:Using the GEPIA2 database,we examined the expression of LINC00894 in lung adenocarcinoma tissues and its relationship with patient prognosis,and the association between LINC00894 and the clinical pathological characteristics of patients was analyzed using the TCGA database.The aim was to utilize real-time fluorescence quantitative PCR(RT-qPCR)to analyze the expression of LINC00894 in both cancerous and adjacent tissues by collecting tissue samples from 20 lung adenocarcinoma patients.The RT-qPCR method was used to detect the presence of LINC00894 in both the human normal bronchial epithelial cell line HBE,as well as the lung adenocarcinoma cell line H1299.H1299 cells were cultured in vitro and divided into Vector group transfected with empty vector pEGFP-C1 and LINC00894 group transfected with overexpressed vector PEGFP-C1-LINC00894.The CCK-8 assay,scratch assay,Transwell assay,and flow cytometry were utilized to measure cell proliferation,migration,invasion,and apoptosis.Results:LINC00894 was low expressed in lung adenocarcinoma tissues and cells and was significantly associated with tumor T stage and primary treatment outcomes[disease progression(PD),disease stabilization(SD),partial response(PR),complete response(CR)].The expression of LINC00894 decreases as both the later T stage and the worse treatment outcome occur in lung adenocarcinoma.After artificially boosting the levels of LINC00894 in vitro,there was a notable reduction in the proliferation,migration,and invasion abilities of the cells,along with an increase in apoptosis.Conclusion:The occurrence and development of lung adenocarcinoma may be strongly influenced by LINC00894.Elevated expression of LINC00894 in lung adenocarcinoma cells inhibits their growth,invasion,and migration,while also inducing apoptosis.
作者 赵松 米良 郑竞雄 孙龙飞 贾佩霖 朱翠敏 梁宗英 ZHAO Song;MI Liang;ZHENG Jingxiong;SUN Longfei;JIA Peilin;ZHU Cuimin;LIANG Zongying(Department of Thoracic Surgery,Affiliated Hospital of Chengde Medical College,Hebei Chengde 067000,China;Department of Oncology Chemoradiotherapy,Affiliated Hospital of Chengde Medical College,Hebei Chengde 067000,China)
出处 《现代肿瘤医学》 CAS 2024年第18期3399-3405,共7页 Journal of Modern Oncology
基金 河北省自然科学基金资助项目(编号:H2021406045) 河北省医学科学研究课题计划(编号:20220411)。
关键词 肺腺癌 LINC00894 增殖 侵袭 迁移 凋亡 lung adenocarcinoma LINC00894 proliferation invasion migration apoptosis
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