DPP3在口腔鳞状细胞癌中的表达及生物学作用

The expression and biological role of DPP3 in oral squamous cell carcinoma

目的:探讨二肽基肽酶3(dipeptidyl peptidase Ⅲ,DPP3)在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达及生物学作用。方法:利用生物信息学技术分析DPP3在OSCC中的表达差异及其对OSCC的诊断和预后价值;qRT-PCR检测DPP3 mRNA在OSCC组织中的表达情况;利用小干扰RNA技术(small interfering RNA,siRNA)在CAL27、SCC15细胞系中敲减DPP3,qRT-PCR和Western Blot实验验证敲减效率;敲减DPP3后通过CCK8实验、EdU实验、划痕实验、Transwell迁移实验和流式细胞术检测CAL27和SCC15细胞的增殖、迁移能力和凋亡率;Western blot实验检测上述两个细胞系中p65及磷酸化p65蛋白(p-p65)的表达。结果:DPP3在OSCC中表达升高(P<0.05);ROC曲线下面积(the area under the ROC curve,AUC)为0.903(95%CI:0.860~0.947),灵敏度和特异度分别为81.5%和90.6%;生存分析结果显示,与DPP3低表达组相比,高表达组患者生存率低、预后差(P<0.05);敲减DPP3后,CAL27、SCC15细胞的增殖和迁移能力下降,凋亡率上升(P<0.05),p65/GAPDH比值无明显变化(P>0.05),p-p65/p65比值降低(P<0.05)。结论:DPP3在OSCC组织中表达升高,对于OSCC具有诊断和预后意义,可以促进OSCC细胞的增殖、迁移能力,抑制细胞凋亡,可能通过NF-κB信号通路发挥相关作用。

Objective:To investigate the expression and biological role of dipeptidyl peptidase Ⅲ(DPP3)in oral squamous cell carcinoma(OSCC).Methods:By utilizing bioinformatics techniques,the differential expression of DPP3 in OSCC and its diagnostic and prognostic value were analyzed.qRT-PCR was used to detect the expression of DPP3 mRNA in OSCC tissues.By employing small interfering RNA(siRNA)technology to knock down DPP3 in CAL27,SCC15 cell lines,the knockdown efficiency was validated through qRT-PCR and Western Blot experiments.After DPP3 knockdown,CCK8,EdU,scratch,transwell migration assays,and flow cytometry were used to assess proliferation,migration capabilities,and apoptosis rates of CAL27 and SCC15 cells.Western Blot experiment to detect the expression of p65 and phosphorylated p65 protein(p-p65)in the aforementioned cell lines.Results:DPP3 expression elevated in OSCC(P<0.05).The area under the ROC curve(AUC)was 0.903(95%CI:0.860~0.947),with sensitivity and specificity of 81.5%and 90.6%,respectively.Survival analysis revealed lower survival rates and poorer prognosis in the high-expression group compared to the low-expression group(P<0.05).After DPP3 knockdown,proliferation and migration abilities of CAL27,SCC15 cells decreased,while apoptosis rates increased(P<0.05).The p65/GAPDH ratio showed no significant change(P>0.05),while the p-p65/p65 ratio decreased(P<0.05).Conclusion:DPP3 is upregulated in OSCC tissues,holding diagnostic and prognostic significance,promoting proliferation and migration capabilities while inhibiting apoptosis in OSCC cells,potentially functioning through the NF-κB signaling pathway.