急性心肌梗死患者外周血内皮细胞微粒中miR-126与ICAM-1的诊断价值研究

Study on diagnostic value of miR-126 and ICAM-1 in peripheral blood endothelial cell particles of patients with acute myocardial infarction

目的研究急性心肌梗死(AMI)患者的外周血内皮细胞微粒(EMPs)中的微小RNA-126(miR-126)与细胞间黏附分子-1(ICAM-1)的诊断价值。方法选取2021年9月至2022年9月该院明确诊断为AMI患者45例作为AMI组,另从同一年龄段、性别的健康体检者中选取45例作为对照组。在进行冠状动脉造影之前,收集患者的外周血,采用流式细胞术对EMPs进行定性和定量分析。分别采用荧光定量PCR和ELISA检测EMPs中的miR-126和ICAM-1表达水平。采用受试者工作特征(ROC)曲线评估相关指标对AMI的诊断价值。结果两组研究对象在性别、年龄、BMI一般资料方面比较,差异无统计学意义(P>0.05);两组PLT、尿素氮(BUN)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白-胆固醇(HDL-C)等生化指标比较,差异无统计学意义(P>0.05)。AMI组miR-126表达水平低于对照组,ICAM-1表达水平高于对照组,差异有统计学意义(P<0.05)。多因素logistic回归结果显示,miR-126、ICAM-1及CK-MB与AMI的发生存在独立相关性(P<0.05)。miR-126对AMI诊断的ROC曲线AUC为0.813(95%CI:0.725~0.903,P<0.001),ICAM-1为0.848(95%CI:0.764~0.933,P<0.001),miR-126与ICAM-1联合诊断的AUC为0.922(95%CI:0.870~0.946,P<0.001)。结论miR-126和ICAM-1在EMPs中的表达水平和AMI存在独立相关性,两者对AMI均有诊断价值。

Objective To study the diagnostic value of miR-126 and ICAM-1 in peripheral blood endothelial microparticles(EMPs)of the patients with acute myocardial infarction(AMI).Methods A total of 45 patients with definitely diagnosed AMI in this hospital from September 2021 to June 2022 were selected as the AMI group.Other 45 healthy subjects in the same age group and sex were selected as the control group.Before coronary angiography,the patient’s peripheral blood was collected,and the flow cytometry was used to conduct the qualitative and quantitative analysis on E MPs.The expression levels of miR-126 and ICAM-1 in EMPs were determined by using fluorogenic quantitative PCR and ELISA.The receiver operating characteristic(ROC)curve was adopted to evaluate the diagnostic value of the related indicators for AMI.Results There was no statistically significant difference in the general data such as age,gender and BMI between the two groups(P>0.05).The biochemical indicators such as PLT,BUN,TC,TG and HDL-C had no statistical difference between the two groups(P>0.05).The expression level of miR-126 in the AMI group was significantly lower than that in the control group,the expression level of ICAM-1 in the AMI group was higher than that in the control group,and the differences were not statistically significant(P<0.05).The miR-126 expression level in the AMI group was lower than that in the control group,the ICAM expression level was higher than that in the control group,and the differences were statistically significant(P<0.05).The multivariate logistic regression results showed that miR-126,ICAM-1 and CK-MB had the independent correlation with the AMI occurrence(P<0.05).The area under the curve(AUC)of mir-126 for diagnosing AMI was 0.813(95%CI:0.725-0.903,P<0.001),which of ICAM-1 was 0.848(95%CI:0.764-0.933,P<0.001),and which of miR-126 and ICAM-1 combination diagnosis was 0.922(95%CI:0.870-0.946,P<0.001).Conclusion The miR-126 and ICAM-1 expression levels in EMPs have the independent correlation with AMI,and the both have the diagnostic value for AMI.