宫颈癌患者血清核苷酸结合寡聚化结构域样受体蛋白3炎症小体和相关细胞因子表达及临床意义

Expression of Serum Nucleotide-Binding Oligomeric Domain-Like Receptor Protein 3 Inflammasome and Related Cytokines in Patients with Cervical Cancer and Its Clinical Significance

目的探究宫颈癌患者血清核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)炎症小体和相关细胞因子的表达水平及临床意义。方法选取2021年3月至2024年3月收治的宫颈癌患者78例作为癌变组,选取同期宫颈上皮内瘤变患者78例作为癌前病变组。比较2组血清NLRP3炎症小体[NLRP3、凋亡相关斑点样蛋白(ASC)、半胱氨酸天冬氨酸蛋白酶-1(Caspase-1)]、白细胞介素-18(IL-18)和白细胞介素-1β(IL-1β)水平;采用Pearson相关性分析血清NLRP3、ASC、Caspase-1与IL-18、IL-1β的关系;分析宫颈癌患者相关细胞因子水平和临床病理特征之间的关系。结果癌变组血清IL-18、IL-1β、NLRP3、ASC、Caspase-1水平均高于癌前病变组(P<0.01);Pearson相关性分析结果显示,患者血清NLRP3、ASC、Caspase-1水平与IL-18和IL-1β水平均呈正相关(P<0.05,P<0.01)。宫颈癌患者肿瘤临床分期越高、分化程度越低,IL-18和IL-1β水平越高(P<0.01);有淋巴结转移和无淋巴结转移的宫颈癌患者IL-18和IL-1β水平比较差异无统计学意义(P>0.05)。结论宫颈癌患者血清IL-18、IL-1β水平和血清NLRP3炎症小体均异常升高,且其水平变化与肿瘤恶性进展有关。

Objective To investigate the expression level and clinical significance of serum nucleotide-binding oligomeric domain-like receptor protein 3(NLRP3)inflammatome and related cytokines in patients with cervical cancer.Methods Seventy-eight patients with cervical cancer admitted from March 2021 to March 2024 were selected as the cancer group,and 78 patients with cervical intraepithelial neoplasia during the same period were selected as the precancer group.The levels of NLRP3 inflammasome[NLRP3,apoptosis-related speck-like protein containing a CARD(ASC),Caspase-1],interleukin-18(IL-18)and interleukin-1β(IL-1β)in serum of the two groups were compared.The correlations of serum NLRP3,ASC,Caspase-1 with IL-18 and IL-1βwere analyzed by Pearson correlation.The relationship between the levels of related cytokines and clinicopathological features in patients with cervical cancer was analyzed.Results The levels of IL-18,IL-1β,NLRP3,ASC,Caspase-1 in cancer group were higher than those in precancer group(P<0.01).The results of Pearson correlation analysis showed that serum NLRP3,ASC,and Caspase-1 levels were positively correlated with IL-18 and IL-1βlevels(P<0.05,P<0.01).The higher the clinical stage and the lower the differentiation degree,the higher the levels of IL-18 and IL-1β(P<0.01).There was no significant difference in IL-18 and IL-1βlevels in cancer patients with and without lymph node metastasis(P>0.05).Conclusion The levels of serum IL-18,IL-1βand NLRP3 inflammasomes are abnormally increased in patients with cervical cancer,and their changes are correlated with malignant progression of cancer.