结直肠癌患者血清IRF5和SOCS-3表达水平对术后肠梗阻的预测价值

Predictive Value of Serum IRF5 and SOCS-3 Expression Levels in Colorectal Cancer Patients for Postoperative Intestinal Obstruction

目的观察结直肠癌患者血清干扰素调节因子-5(interferon regulatory factor-5,IRF5)、细胞因子信号转导抑制因子-3(suppressors of cytokine signaling-3,SOCS-3)水平,并探讨其水平对患者术后肠梗阻发生的预测价值。方法选取2022年1月~2023年3月在沧州市人民医院就诊的100例结直肠癌患者为研究对象,根据患者术后是否发生肠梗阻,将其分为肠梗组(n=14)和对照组(n=86)。酶联免疫吸附(ELISA)法检测术前血清IRF5和SOCS-3水平。采用Logistic回归分析结直肠癌患者术后肠梗阻发生的影响因素。采用受试者工作特征(ROC)曲线分析血清IRF5,SOCS-3水平对结直肠癌患者术后肠梗阻发生的诊断价值。结果肠梗组患者血清IRF5水平(0.68±0.09ng/ml)低于对照组(0.89±0.12ng/ml),血清SOCS-3水平(97.23±15.94pg/ml)高于对照组(75.03±12.46pg/ml),差异具有统计学意义(t=6.257,5.937,均P<0.05)。SOCS-3是影响结直肠癌患者术后肠梗阻发生的独立危险因素(OR=2.197,95%CI:1.167~4.138,P<0.05),IRF5是影响结直肠癌患者术后肠梗阻发生的独立保护因素(OR=0.823,95%CI:0.705~0.961,P<0.05)。血清IRF5,SOCS-3以及二者联合诊断结直肠癌患者术后肠梗阻的ROC曲线下面积(AUC)分别为0.852(95%CI:0.767~0.915),0.817(95%CI:0.727~0.887)和0.953(95%CI:0.891~0.985),二者联合诊断效能高于血清IRF5,SOCS-3单独检测效能,差异具有统计学意义(Z=1.967,2.034,P=0.049,0.042)。结论结直肠癌患者血清IRF5水平降低,SOCS-3水平升高,且二者对结直肠癌患者发生术后肠梗阻具有一定的预测价值。

Objective To observe serum interferon regulatory factor-5(IRF5)and suppressors of cytokine signaling-3(SOCS-3)levels in patients with colorectal cancer,and investigate predictive value of their levels on the occurrence of postoperative intestinal obstruction in patients.Methods A total of 100 colorectal cancer patients who attended the Cangzhou People’s Hospital from January 2022 to March 2023 were selected as the study subjects.According to whether the patient experienced intestinal obstruction after surgery,they were divided into the intestinal obstruction group(n=14)and the control group(n=86).Preoperative serum levels of IRF5 and SOCS-3 were detected by enzyme-linked immunosorbent assay(ELISA).Factors influencing the occurrence of postoperative intestinal obstruction in colorectal cancer patients were analyzed by Logistic regression.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of serum IRF5 and SOCS-3 levels on the occurrence of postoperative intestinal obstruction in colorectal cancer patients.Results Serum IRF5 levels in the intestinal infarction group(0.68±0.09ng/ml)were lower,but serum SOCS-3 levels in the intestinal infarction group(97.23±15.94pg/ml)were higher than in the control group(0.89±0.12ng/ml,75.03±12.46pg/ml),with significant differences(t=6.257,5.937,all P<0.05).SOCS-3 was an independent risk factor(OR=2.197,95%CI:1.167~4.138,P<0.05),and IRF5 was an independent protective factor(OR=0.823,95%CI:0.705~0.961,P<0.05)affecting the occurrence of postoperative bowel obstruction in patients with colorectal cancer.The areas under the curve(AUC)of serum IRF5,SOCS-3,and the combination of the two for the diagnosis of postoperative intestinal obstruction in colorectal cancer patients were 0.852(95%CI:0.767~0.915),0.817(95%CI:0.727~0.887)and 0.953(95%CI:0.891~0.985),respectively.The combined diagnostic efficacy of the serum IRF5,SOCS-3 was higher than the efficacy of the test alone,with significant differences(Z=1.967,2.034,P=0.049,0.042).Conclusion Serum IRF5 levels are reduced,but SOCS-3 levels are increased in patients with colorectal cancer.Both have a predictive value for the development of postoperative bowel obstruction in patients with colorectal cancer.