非肌层浸润性膀胱癌组织中OTUD5和RNF186表达与临床病理特征的关系及预后价值研究

Study on the Clinicopathological Characteristics and Prognostic Value of the Expression of OTUD5 and RNF186 in Non-muscle Invasive Bladder Cancer

目的研究非肌层浸润膀胱癌(non-muscle invasive bladder cancer,NMIBC)癌组织中结构域蛋白去泛素化酶5(domain protein ubiquitin 5,OTUD5)、环指蛋白186(ring finger protein 186,RNF186)表达与临床病理特征的关系及预后价值研究。方法收集2019年1月~2020年1月唐山市中医院收治的106例NMIBC患者。免疫组织化学检测NMIBC癌组织及癌旁组织OTUD5,RNF186表达。比较不同临床病理特征NMIBC癌组织OTUD5,RNF186表达差异。Kaplan-Meier曲线分析(Log-Rank检验)OTUD5,RNF186表达对NMIBC无进展生存预后的影响。COX回归模型分析影响NMIBC患者无进展生存预后的因素。结果NMIBC癌组织中OTUD5(62.26%),RNF186(66.04%)的阳性率高于癌旁组织(11.32%,8.49%),差异具有统计学意义(χ^(2)=59.146,75.079,均P<0.05)。NMIBC癌组织中OTUD5与RNF186表达呈显著正相关(r=0.659,P<0.05)。T 1期、高级别尿路上皮癌NMIBC癌组织中OTUD5(72.97%,84.21%),RNF186(77.03,86.84%)阳性率高于Ta/Tis期(37.50%,50.00%)、低级别尿路上皮癌(40.63%,54.41%),差异具有统计学意义(χ^(2)=11.964,13.199;12.143,11.431,均P<0.05)。OTUD5阳性组(40.91%)、RNF186阳性组(45.71%)三年累积无进展生存率低于OTUD5阴性组(90.00%)、RNF186阴性组(86.11%)患者(Log-Ranktestχ^(2)=24.710,14.586,均P<0.05)。OTUD5阳性(OR=1.446,95%CI:1.086~1.925)、RNF186阳性(OR=1.579,95%CI:1.132~2.024)、肿瘤TNM分期T1期(OR=1.582,95%CI:1.219~2.054)、病理分级高级别(OR=1.570,95%CI:1.188~2.074)是影响NMIBC患者无进展生存的独立危险因素(均P<0.001)。结论NMIBC患者癌组织OTUD5,RNF186表达升高,与肿瘤TNM分期、病理分级有关,是影响NMIBC患者预后的独立危险因素。

Objective To study the expression of domain protein ubiquitin 5(OTUD5)and ring finger protein 186(RNF186)in non-muscle invasive bladder cancer(NMIBC)and their relationship with clinicopathological characteristics and prognostic value.Methods A total of 106 NMIBC patients admitted to Tangshan Traditional Chinese Medicine Hospital from January 2019 to January 2020 were collected.OTUD5 and RNF186 expression in NMIBC cancer tissue and para-cancer tissue were detected by immunochemistry.The differences between OTUD5 and RNF186 expressions in NMIBC cancer tissues with different clinical and pathological characteristics were compared.Kaplan-Meier curve analysis(Log Rank test)was used to analyze the impact of OTUD5 and RNF186 expression on progression free survival of NMIBC.COX regression model analysis was used to analyze factors affecting progression free survival in NMIBC patients.Results The positive rates of OTUD5(62.26%)and RNF186(66.04%)in NMIBC cancer tissue were higher than those in adjacent tissues(11.32%,8.49%),with significant differences(χ^(2)=59.146,75.079,all P<0.05).There was a significant positive correlation between OTUD5 and RNF186 expressions in NMIBC cancer(r=0.659,P<0.05).The positive rates of OTUD5(72.97%,84.21%)and RNF186(77.03%,86.84%)in T1 stage and high-grade NMIBC cancer tissues were higher than those in Ta/Tis stage(37.50%,50.00%)and low-grade cancer tissues(40.63%,54.41%),and the differences were significant(χ^(2)=11.964,13.199;12.143,11.431,all P<0.05).The 3-year cumulative progression free survival rate of patients in the OTUD5 positive group(40.91%)and RNF186 positive group(45.70%)were lower than that in the OTUD5 negative group(90.00%)and RNF186 negative group(86.11%)(Log Rank testχ^(2)=24.710,14.586,all P<0.05).OTUD5 positive(OR=1.446,95%CI:1.086~1.925),RNF186 positive(OR=1.579,95%CI:1.132~2.024),Tumor TNM stage T1(OR=1.582,95%CI:1.219~2.054)and high pathological grade(OR=1.570,95%CI:1.188~2.074)were independent risk factors affecting progression free survival in NMIBC patients(all P<0.001).Conclusion The increased expression of OTUD5 and RNF186 in cancer tissue of NMIBC patients are related to tumor TNM staging and pathological grading and are independent risk factors affecting the prognosis of NMIBC patients.