虾源肌球蛋白抗原表位的计算机辅助计算

Computer-Aided Calculation of Antigen Epitope of Tropomyosin in Shrimp

利用在线软件和线下软件对来源于刀额新对虾(Metapenaeus ensis)的过敏原Met e1的B细胞抗原表位和T细胞抗原表位进行预测、筛选以及辅助计算.首先,通过uniprot蛋白数据库获取Met e1蛋白质氨基酸序列;其次,用ExPASY ProtParam, SignalP-5.0 Server和TMHMM Server v.2.0在线工具对Met e1的理化性质、信号肽与跨膜区域进行分析,用PSIPRED在线工具、 SOPMA在线工具和DNAstar软件联合预测计算Met e1二级结构,用Swiss model在线软件预测计算Met e1三级结构;再次,用DNAStar线下软件和IEDB在线软件综合预测计算线性B细胞表位,用IEDB在线软件预测计算CD4+T细胞表位和CD8+T细胞表位;最后将所得结果进行B细胞表位和T细胞表的筛选.结果表明:Met e1蛋白的B细胞表位为15~28, 45~49, 92~95, 125~130, 150~153, 255~258位氨基酸;T细胞表位为78~84, 223~232位氨基酸.

Online and offline software were used to predict,screen and assist in the calculation of the B-cell and T-cell epitopes of the allergen Met e1 from Metapenaeus ensis.Firstly,the amino acid sequence of Met e1 protein was retrieved from the uniprot protein database.Secondly,the physicochemical properties,signal peptides,and transmembrane regions of Met e1 were analyzed by using online tools ExPASY ProtParam,SignalP-5.0 Server,and TMHMM Server v.2.0.The secondary structure of Met e1 was jointly predicted and calculated by using the PSIPRED online tool,SOPMA online tool,and DNAstar software,while the tertiary structure of Met e1 was predicted and calculated by using Swiss model online software.Thirdly,the linear B-cell epitopes were comprehensively predicted and calculated by using DNAStar offline software and IEDB online software,CD4+T and CD8+T-cell epitopes were predicted and calculated by using the IEDB online software.Finally,the obtained results were screened for B-cell and T-cell epitopes.The results show that the B-cell epitopes of Met e1 protein are located at amino acids 15—28,45—49,92—95,125—130,150—153 and 255—258,and T-cell epitopes are located at amino acids 78—84 and 223—232.