益生菌固态发酵独活茎叶工艺的优化

Optimization of probiotic solid-state fermentation process for stems and leaves of Angelica pubescens Radix

试验旨在优化益生菌固态发酵独活茎叶工艺,提高独活茎叶中蛇床子素的含量。试验采用解淀粉芽孢杆菌SSYB发酵独活茎叶,在单因素试验的基础上,通过正交试验设计优化固态发酵工艺,采用高效液相色谱法测定发酵前后独活茎叶中蛇床子素的含量。结果显示,优化的独活茎叶发酵工艺为发酵时间72 h、发酵温度37℃、接种量5%、含水量65%。在该条件下,独活茎叶中蛇床子素含量为1.81 mg/g,比发酵前极显著提高了45.97%(P<0.01)。研究表明,通过优化解淀粉芽孢杆菌SSYB固态发酵独活茎叶的工艺,提高了独活茎叶中蛇床子素的含量。

The purpose of this experiment was to optimize the process of probiotics solid-state fermentation on stems and leaves of Angelica pubescens Radix,and to increase the content of osthole in stems and leaves of Angelica pubescens Radix.In this experiment,Bacillus amyloliquefaciens strain SSYB was used to ferment the stems and leaves of Angelica pubescens Radix,and on the basis of single factor test,the solid-state fermentation process was optimized by orthogonal experimental design,and the content of osthole in stems and leaves of Angelica pubescens Radix before and after the fermentation process was determined by high performance liquid chromatography.The results showed that the optimized fermentation process of stems and leaves of Angelica pubescens Radix was fermentation time of 72 h,fermentation temperature of 37℃,inoculation amount of 5%,and water content 65%.Under these conditions,the content of osthole in stems and leaves of Angelica pubescens Radix was 1.81 mg/g,which was significantly higher than that before fermentation by 45.97%(P<0.01).The results show that the content of osthole in stems and leaves of Angelica pubescens Radix is increased by optimizing the solid-state fermentation process of Bacillus amyloliquefaciens SSYB.