枯草芽孢杆菌JTFM1001在防控玉米黄曲霉菌中的应用

Application of Bacillus subtilis JTFM1001 in controlling Aspergillus flavus of corn

试验旨在寻找能够防控玉米黄曲霉菌的生物制剂。从不同省份的健康饱满玉米籽粒中,分离拮抗菌并验证其抑菌效果。结果显示,从河北玉米籽粒中分离获得1株对黄曲霉菌具有明显抑制效果的菌株JTFM1001,室内对峙拮抗试验中抑制率达87.5%。活体抑菌解毒试验和田间防治试验证实,该菌株抑菌能力较强,且对植株具有一定的促生作用,田间促生率和抑毒率分别为6.5%和55.0%。菌株JTFM1001的发酵代谢产物和蛋白提取物能够较好地抑制黄曲霉菌生长。菌株JTFM1001鉴定为枯草芽孢杆菌。研究表明,枯草芽孢杆菌JTFM1001具有开发成生防菌剂的实际应用价值。

The experiment aimed to find a biological preparation that can control Aspergillus flavus in corn.Antagonistic bacteria were isolated from healthy and full corn kernels from different provinces and their antibacterial effects were verified.The results showed that a strain JTFM1001 with a significant inhibitory effect on Aspergillus flavus was isolated from corn kernels in Hebei Province,with an inhibition rate of 87.5%in indoor confrontational antagonism tests.The in vivo antibacterial detoxification tests and field control experiments confirmed that this strain had strong antibacterial ability and a certain promoting effect on the growth of plants,with the field promotion rate and detoxification rate being 6.5%and 55.0%,respectively.The fermentation metabolites and protein extracts of strain JTFM1001 can effectively inhibit the growth of Aspergillus flavus.Strain JTFM1001 was identified as Bacillus subtilis.The study indicates that Bacillus subtilis JTFM1001 has practical application value in the development of biocontrol agents.