丙泊酚对阿霉素致大鼠心肌毒性的影响

Effect of propofol on doxorubicin-induced cardiotoxicity in rats

目的评价丙泊酚对阿霉素致大鼠心肌毒性的影响。方法SPF级健康雄性SD大鼠30只,2~3月龄,体质量200~220 g,采用随机数字表法分为3组(n=10):对照组(C组)、阿霉素组(D组)和丙泊酚组(P组)。D组和P组腹腔注射阿霉素2.5 mg/kg,依据每日大鼠体质量调整给药剂量,隔日1次,共6次,制备阿霉素致大鼠心肌毒性模型,P组在心肌毒性模型制备成功后腹腔注射丙泊酚50 mg/kg。注射丙泊酚后2 h时麻醉大鼠心脏取血,采用ELISA法测定大鼠血清cTnI和CK-MB浓度;随后处死大鼠取心肌组织,光镜下观察心肌组织病理学结果;透射电镜下观察心肌组织,测定心肌组织线粒体体积分数、表面积密度、表面积/体积比、平均面积和数密度。结果与C组相比,D组和P组血清cTnI和CK-MB浓度升高,线粒体体积分数、表面积密度和数密度降低(P<0.05);与D组相比,P组血清cTnI和CK-MB浓度降低,线粒体体积分数、表面积密度和数密度升高(P<0.05);3组线粒体表面积/体积比和平均面积比较差异无统计学意义(P>0.05)。结论丙泊酚可减轻阿霉素致大鼠心肌毒性。

ObjectiveTo evaluate the effect of propofol on doxorubicin-induced cardiotoxicity in rats.MethodsThirty SPF healthy male Sprague-Dawley rats,aged 2-3 months,weighing 200-220 g,were divided into 3 groups(n=10 each)by the random number table method:control group(C group),doxorubicin group(D group)and propofol group(P group).The rat cardiotoxicity model was prepared by intraperitoneal injection of doxorubicin 2.5 mg/kg,and the administered dose was adjusted according to the daily body weight once every other day for a total of 6 times in D and P groups.Propofol 50 mg/kg was intraperitoneally injected after successful preparation of the cardiotoxicity model in P group.Blood samples were taken from the heart of the anesthetized rats at 2 h after propofol injection for determination of the concentrations of serum cardiac troponin I(cTnI)and creatine kinase-MB(CK-MB)by enzyme-linked immunosorbent assay.Then the rats were sacrificed,and the myocardial tissues were obtained for microscopic examination of the pathological changes(with a light microscope)and for determination of the mitochondrial volume fraction,surface-area density,surface-area-to-volume ratio,mean mitochondrial area and the number density(by transmission electron microscopy).ResultsCompared with C group,the concentrations of cTnI and CK-MB were significantly increased,and the mitochondrial volume fraction,surface-area density,and the number density were decreased in D and P groups(P<0.05).Compared with D group,the concentrations of cTnI and CK-MB were significantly decreased,and the mitochondrial volume fraction,surface-area density,and the number density were increased in P group(P<0.05).There was no significant difference in the surface-area-to-volume ratio and mean mitochondrial area among the 3 groups(P>0.05).ConclusionsPropofol can attenuate doxorubicin-induced myocardial toxicity in rats.