过表达AtAUR1对UV-B胁迫下根尖细胞周期调控的影响

Effect of Overexpression of AtAUR1 on Cell Cycle Regulation in Root Tips under UV-B Stress

随着南北两极臭氧层空洞的持续增大,UV-B辐射对整个地球的生态系统造成的危机不断增强.植物受其特殊生活方式的影响,更容易遭受增强UV-B辐射的影响.UV-B辐射作为一种非生物压力信号会造成植物细胞DNA的损伤从而显著抑制植物生长,严重时甚至会导致植物的死亡.Aurora激酶作为调控有丝分裂的重要蛋白,在有丝分裂过程中参与了染色体的定位、分离以及胞质分裂等过程,实验室之前的研究结果表明过表达AUR1可以缓解UV-B辐射导致的损伤.研究以模式植物拟南芥为材料,通过有性杂交的方法获得含CYCB1;1-GUS标签的拟南芥AUR1过表达株系.通过GUS染色和RT-qPCR的方法对获得的转基因株系在UV-B处理后CYCB1;1的组织表达模式进行了分析,实验结果证明了过表达AUR1可以上调植物根尖细胞中CYCB1;1的基因表达,UV-B辐射处理后的染色结果进一步证明了AUR1参与了UV-B辐射后根尖有丝分裂周期的调控,为揭示过表达AUR1对根尖有丝分裂周期调控的影响提供了一定的理论基础.

As the hole in the ozone layer in the North and South Poles continues to grow,the crisis caused by UV-B radiation to the ecosystems of the entire planet is growing.Plants are affected by their particular lifes-tyles and are more susceptible to the effects of enhanced UV-B radiation.As an abiotic stress signal,UV-B radi-ation can cause damage to the DNA of plant cells,which can significantly inhibit plant growth,and even lead to plant death in severe cases.As an important protein in the regulation of mitosis,Aurora kinase is involved in the localization and separation of chromosomes and cytokinesis during mitosis,and previous laboratory studies have shown that overexpression of AUR1 can alleviate the damage caused by UV-B radiation.In this study,Arabidop-sis AUR1 overexpressing lines containing the CYCB1;1-GUS tag was obtained by sexual hybridisation.The tissue expression pattern of CYCB1;1 in the obtained transgenic lines after UV-B treatment was analysed by GUS stai-ning and RT-qPCR,and the experimental results proved that overexpression of AUR1 could up-regulate the gene expression of CYCB1;1 in the root tip cells of the plants,and the staining results after UV-B radiation treatment further proved that AUR1 was involved in the UV-B radiation root tip.The staining results after UV-B radiation treatment further proved that AUR1 was involved in the regulation of mitotic cycle in root tips after UV-B radia-tion,which provided a theoretical basis for revealing the effect of overexpression of AUR1 on the regulation of mi-totic cycle in root tips.