高效液相色谱法测定黑老虎根中内消旋二氢愈创木脂酸含量

Determination of meso - Dihydroguaiaretic Acid in Kadsura Coccinea Root by HPLC

目的 建立测定黑老虎根乙酸乙酯提取物(ET)及抗炎有效部位ET5中内消旋二氢愈创木脂酸(meso-DGA)含量的高效液相色谱法。方法 色谱柱为BDS Hypersil C18柱(150 mm×4.6 mm,5μm),流动相为0.1%甲酸水溶液-乙腈(梯度洗脱),流速为1.0 m L/min,检测波长为282 nm,柱温为45℃,进样量为10μL。结果 meso-DGA质量浓度在1.0~50.0μg/m L范围内与峰面积线性关系良好(R2=0.999 4,n=6);精密度、稳定性、重复性试验结果的RSD均不大于2.0%;平均加样回收率为100.08%,RSD为1.02%(n=6)。结论 所建立的方法操作简便、重复性好、结果准确,可用于测定黑老虎根ET及ET5中meso-DGA的含量。

Objective To establish a high-performance liquid chromatography method for determining the content of meso-dihydroguaiaretic acid(meso-DGA)in the ethyl acetate extract(ET)and anti-inflammatory active fraction ET5 of Kadsura coccinea root.Methods The chromatographic column was the BDS Hypersil C18 column(150 mm×4.6 mm,5μm),the mobile phase was 0.1%formic acid aqueous solution-acetonitrile(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 282 nm,the column temperature was 45℃,the injection volume was 10μL.Results The linear range of meso-DGA was 1.0-50.0μg/mL(R2=0.9994,n=6).The RSDs of precision,stability and repeatability tests were not greater than 2.0%.The average recovery rate of meso-DGA was 100.08%,with an RSD of 1.02%(n=6).Conclusion The established method is easy,repeatable and accurate,which can be used to determine the content of meso-DGA in ET and ET5 of Kadsura coccinea root.