中微量元素复合菌剂制备及其对燕麦发芽和幼苗生长的影响

Preparation of a medium and trace elements compound bacterial agent and its effects on oat germination and seedling growth

【目的】研究中微量元素混合物添加对复合促生菌剂的效果及影响。【方法】将不同浓度中微量元素混合物添加至复合促生菌剂,研究对菌剂的影响并筛选不同中微量元素混合物的最佳添加量,同时通过发芽试验研究菌剂对燕麦种子萌发和幼苗生长的影响。【结果】促生菌剂添加微量元素混合物T2,T3,T4在添加量分别为2.0‰,1.5‰,0.5‰,中量元素混合物M1添加量在1.0%时效果最好,其有效活菌数在3.73×10^(8)~4.70×10^(8) cfu/mL,pH值介于8.31~8.45。发芽试验表明,复合促生菌剂原液对燕麦种子的发芽具有显著抑制作用,添加微量元素混合物后均可缓解该抑制作用,相较于原液发芽率最高可提升52.78%;稀释一定浓度后可显著提高种子发芽率、发芽势、发芽指数及活力指数,其中菌剂W3在稀释100倍后发芽率最高,为72.50%,较CK提升了61.11%。【结论】促生菌剂可促进燕麦幼苗根长、苗长增加,提高地上地下生物量(鲜重和干重),其中添加微量元素混合物T2的菌剂W2在稀释100倍时对地下生物量(鲜重和干重)促进效果最好,添加微量元素混合物T3的菌剂W3在稀释100倍时对地上生物量(鲜重和干重)促进效果最好。

【Objective】To investigate the effectsof adding medium and trace elements to acompound growth-pro-moting agent and their influence on its efficacy.【Method】Mixtures of medium and trace elements at various concen-trations were added to the compound growth-promoting agent to assess their impactand determine the optimal dos-age.The effects of the agent on oat seed germination and seedling growth were were evaluated through germination tests.【Result】The trace element mixtures T2,T3 and T4 were at concentrations of 2.0‰,1.5‰and 0.5‰,respec-tively,while the medium element mixture M1 was at 1.0%.The effective viable bacterialcount ranged from 3.73×10^(8) to 4.70×10^(8) cfu/mL,and the pH value ranged from 8.31 to 8.45.The germination test revealed that the stock solution of the compound growth promoting agent significantly inhibited oat seed germination.However,the addition of trace elements mixtures alleviated this inhibitory effect,increasing the germination rate by up to 52.78%compared to the stock solution.Dilution of the agent improved the germination rate,germination potential,germination index and vigor index of seeds.Among the treatments,the germination rate of bacterial agent W3 was the highest after 100-fold dilution,reaching 72.50%,which was 61.11%higher than the control(CK).【Conclusion】The bacteria agent can promote oat seedlinggrowth,increasing root length,shoot length,and improving underground biomass(both fresh and dry weight).The best results for underground biomass were achieved with T2 inoculant W2 at a 100-fold dilution,while T3 inoculant W3 at a 100-fold dilution was most effective for aboveground biomass.This study provides a theoretical basis for optimizing the early application of bacterial agents to enhance their effectiveness.