摘要
目的建立转运蛋白颗粒复合物亚基11(Trappc11)诱导型基因敲除小鼠模型。方法和结果在Trappc11基因外显子3~5的两侧分别引入loxP位点,利用CRISPR/Cas9技术获得F0代C57BL/6J小鼠;将通过PCR扩增及测序鉴定阳性的F0代C57BL/6J小鼠与C57BL/6J野生型小鼠交配、繁殖,获得F1代Trappc11flox/+小鼠;再将Trappc11flox/+小鼠与UBC-CreERT2小鼠交配,经过2代繁殖,最终获得Trappc11诱导型全身性基因敲除小鼠模型。结论通过CRISPR/Cas9和Cre-loxP技术成功建立了Trappc11诱导型基因敲除小鼠模型,为揭示Trappc11在多器官系统疾病中的病理生理学作用提供了重要工具。
Objective To establish an inducible knockout mouse model of trafficking protein particle complex subunit 11(Trappc11).Methods and results LoxP sites were introduced on both sides of exon 3-5 of Trappc11,and then the CRISPR/Cas9 technique was used to establish F0 C57BL/6J mice.The positive F0 generation mice were identified by polymerase chain reaction amplification and sequencing.After that,F0 positive mice were mated with C57BL/6J wild type mice to obtain F1 Trappc11flox/+mice.And then,Trappc11flox/+mice were mated with UBC-CreERT2 mice,and finally Trappc11 inducible systemic knockout mouse model was obtained after 2 generations.Conclusion The Trappc11 inducible knockout mouse model is established using CRISPR/Cas9 and Cre-loxP,providing an important tool for revealing the pathophysiological role of Trappc11 in multi-organ system diseases.
作者
王波波
弓梦
温晶
ALUS Xiaoli
李优磊
WANG Bobo;GONG Meng;WEN Jing;ALUS Xiaoli;LI Youlei(Department of Physiology,Medical School of Yan’an University,Yan’an 716000,Shaanxi,China;Diabetes Research Center,Albert Einstein College of Medicine,New York 10461,USA)
出处
《海军军医大学学报》
CAS
CSCD
北大核心
2024年第9期1156-1161,共6页
Academic Journal of Naval Medical University
基金
陕西省教育厅科学研究计划项目(22JK0613)
陕西省自然科学基础研究计划项目(2024JC-YBQN-0949)
延安大学博士科研启动项目(YDBK2021-07)
陕西省高校科协青年人才托举计划(20220217)。