广西四个地方猪的精液品质分析

Semen Quality Analysis of Four Local Pig in Guangxi

为了解广西东山猪、德保猪、隆林猪、桂中花猪新鲜精液和冷冻后精液的质量情况,该试验对4个地方猪集中饲养管理、调教采精,测定和分析采精量、鲜精总精子数、鲜精活力、稀释后活力、冷冻后解冻的活力(冻后活力)等指标。结果表明:冷冻精液解冻后合格率最高的是隆林猪,为97.49%,最低的是桂中花猪,为64.02%。成功采精比例最高的是桂中花猪,为91%,最低的是东山猪40%。德保猪平均采精量极显著高于其他3个地方猪种(P<0.01),东山猪、德保猪、隆林猪平均原精活力极显著高于桂中花猪(P<0.01)。隆林猪平均总精子数极显著高于其他3个地方猪种(P<0.01)。东山猪、隆林猪和德保猪平均冷冻前活力、平均解冻后活力均极显著高于桂中花猪(P<0.01)。东山猪冷冻精液质检平均活力极显著高于德保猪(P<0.01),隆林猪平均前向运动精子数显著高于东山猪(P<0.05)。德保猪平均畸形率极显著高于桂中花猪和隆林猪(P<0.01)。隆林猪和桂中花猪平均菌落数极显著高于德保猪(P<0.01),东山猪平均菌落数显著高于德保猪(P<0.05)。可见,4个不同地方猪的精液质量和耐冻性存在差异,其中隆林猪的鲜精精液品质和耐冻性较好,适合冷冻保存,桂中花猪鲜精精液品质和耐冻性较差,需进一步探索适合的冷冻保存方法。

In order to understand the quality of fresh semen and frozen semen of the Guangxi Dongshan pig,Debao pig,Longlin pig,and Guizhonghua pig,this experiment focused on the feeding and management of four local pigs,adjusted and collected semen,and measured and analyzed the amount of semen collected,the total number of fresh sperm,the vitality of fresh sperm,the vitality after dilution,the vitality after freezing and thawing(the vitality after freezing)and other indicators.The results showed that the highest qualified rate of frozen semen after thawing was 97.49%in Longlin pigs,and the lowest was 64.02%in Guizhong pigs.The highest proportion of successful sperm collection was Guizhonghua pig,which was 91%,and the lowest was Dongshan pig,which was 40%.The average semen collection of the Debao pig was significantly higher than that of the other three local pig breeds(P<0.01).The average sperm motility of Dongshan pig,Debao pig,and Longlin pig was significantly higher than that of Guizhonghua pig(P<0.01).The average total sperm count of the Longlin pig was significantly higher than that of the other three local pig breeds(P<0.01).The average pre-freezing and post-thawing activity of the Dongshan pig,Longlin pig,and Debao pig were significantly higher than those of the Guizhonghua pig(P<0.01).The average motility of frozen semen in Dongshan pigs was significantly higher than that in Debao pigs(P<0.01),and the average number of forward-moving sperm in Longlin pigs was significantly higher than that in Dongshan pigs(P<0.05).The average deformity rate of Debao pigs was significantly higher than that of Guizhonghua pigs and Longlin pigs(P<0.01).The average colony number of the Longlin pig and Guizhonghua pig was significantly higher than that of the Debao pig(P<0.01),and the average colony number of the Dongshan pig was considerably higher than that of the Debao pig(P<0.01).It can be seen that the semen quality and freezing tolerance of four different local pigs are different.Among them,the fresh semen quality and freezing tolerance of Longlin pigs are better and suitable for cryopreservation.The fresh semen quality and freezing tolerance of Guizhonghua pigs are poor,and it is necessary to further explore suitable cryopreservation methods.