气相色谱法测定食品中苯甲酸、山梨酸和脱氢乙酸含量

Determination of Benzoic Acid,Sorbic Acid,and Dehydroacetic Acid Content in Food by Gas Chromatography

目的:建立气相色谱法测定食品中苯甲酸、山梨酸和脱氢乙酸含量的方法。方法:样品经酸化处理后,使用乙醚进行提取,气相色谱仪分离和定量分析。结果:苯甲酸、山梨酸和脱氢乙酸在各自线性范围内线性关系良好,精密度相对标准偏差在2.04%~4.23%,平均回收率在98.77%~100.14%,检出限分别为1.29 mg·kg^(-1)、1.13 mg·kg^(-1)和1.03 mg·kg^(-1),均符合相关要求。结论:本文建立的气相色谱法具有高精密度、高准确度和强选择性的特点,适用于酱油、果汁和腐乳等食品中苯甲酸、山梨酸和脱氢乙酸的测定。

Objective:To establish a gas chromatography method for the determination of benzoic acid,sorbic acid,and dehydroacetic acid in food.Method:The samples were treated with acidification,extracted with ether,and then separated and quantitatively analyzed by gas chromatography.Result:Benzoic acid,sorbic acid and dehydroacetic acid had good linear relationships in their respective linear ranges.The relative standard deviation obtained from precision experiments was between 2.04%and 4.23%,and the recovery rate of spiked samples was between 98.77%and 100.14%,the detection limits were 1.29 mg·kg^(-1),1.13 mg·kg^(-1)and 1.03 mg·kg^(-1).Conclusion:The established gas chromatography method has high precision,accuracy,and strong selectivity,and is suitable for the determination of benzoic acid,sorbic acid and dehydroacetic acid in soy sauce,fruit juice and fermented bean curd.