液相色谱法测定食品中合成着色剂

Determination of Synthetic Colorants in Food by Liquid Chromatography

目的:建立一种液相色谱法测定食品中合成着色剂的方法。方法:用乙醇氨水溶液提取食品中的合成着色剂,经固相萃取柱净化后,用液相色谱仪(配二极管阵列检测器)测定,外标法定量。结果:11种合成着色剂在0.2~10.0 μg·mL^(-1)线性关系良好,线性相关系数为0.999 4~1.000 0,加标回收率为94.2%~109.6%,相对标准偏差在0.55%~5.52%。柠檬黄、喹啉黄、日落黄、胭脂红、新红和赤藓红的检出限均为0.5 mg·kg^(-1),定量限均为1.5 mg·kg^(-1);苋菜红、诱惑红、亮蓝、酸性红和靛蓝的检出限均为0.3 mg·kg^(-1),定量限均为1.0 mg·kg^(-1)。结论:该方法的重复性好、灵敏度高、实用性强,可以同时测定食品中多种合成着色剂的含量。

Objective:To establish a method for the determination of synthetic colorants in food by liquid chromatography.Method:The synthetic colorants in food were extracted with ethanol ammonia solution,purified by solid phase extraction column,determined by liquid chromatography (with diode array detector),and quantified by external standard method.Result:The linear range of 11 synthetic colorants was 0.2~10.0 μg·mL^(-1),the linear correlation coefficient was 0.999 4~1.000 0,the recovery rate was 94.2%~109.6%,and the relative standard deviation was 0.55%~5.52%.The detection limits of tartrazine,quinoline yellow,sunset yellow,carmine,new red and erythrosine were 0.5 mg·kg^(-1),and the limits of quantification were 1.5 mg·kg^(-1).The limits of detection of amaranth,allura red,brilliant blue,acid red and indigo were 0.3 mg·kg^(-1),and the limits of quantitation were1.0 mg·kg^(-1).Conclusion:The method has good repeatability,high sensitivity and strong practicability,and can simultaneously determine the content of various synthetic colorants in food.