负载纳米铂的石墨烯传感器伏安测定阿奇霉素

Voltammetric determination of Azithromycin by using graphene sensor loaded with platinum nanoparticles

采用一步水热法合成了负载纳米铂粒子的聚二烯丙基二甲基氯化铵功能化石墨烯(PtNPs/PDDA/GR),用红外光谱(IR)、X射线衍射(XRD)、电化学交流阻抗(EIS)等手段对复合纳米材料进行了表征.采用循环伏安法研究了阿奇霉素在(PtNPs/PDDA/GR/GCE)传感器上的电化学行为,并优化了电化学测量条件.结果表明,该传感器具有较好的电化学响应,在阿奇霉素浓度为8.0×10^(-7)~1×1.0^(-5) mol·L^(-1)时,其氧化峰电流(I p)与浓度呈良好的线性关系,检出限为3.7×10^(-8) mol·L^(-1).该传感器成功用于市售药品中阿奇霉素含量的测定,回收率为99.4%~101.6%.该方法测定阿奇霉素具有灵敏度高、准确性好等优点,具有一定的实际应用价值.

Platinum nanoparticles/poly-diallyldimethylmmonium chloride/graphene(PtNPs/PDDA/GR)was prepared by an one-pot hydrothermal method.This composite was characterized by Infrared spectrum(IR),X-ray diffraction(XRD),electrochemical impedance spectroscopy(EIS).The electrochemical behavior of Azithromycin at sensor PtNPs/PDDA/GR/GCE in phosphate buffer solution(pH 7.0)was investigated by using cyclic voltammetry(CV),and the electrochemical measurement conditions were also optimized.The results show better electrochemical response of the voltammetric sensor.The oxidation peak current of Azithromycin increases linearly with concentration in the range of 8.0×10^(-7)~1×1.0^(-5) mol·L^(-1),and the detection limit is 3.7×10^(-8) mol·L^(-1).This sensor is employed to determinate of Azithromycin in pharmaceutical preparations,and the recovery is 99.4%~101.6%.The method has the advantages of high sensitivity and good accuracy,and has the value of practical application.