正交实验法优化杜仲叶发酵工艺

Optimization of Fermentation Process of Eucommia ulmoides Leaves by Orthogonal Experiment

以杜仲叶为原料,采用酵母菌进行发酵,利用单因素实验、正交实验结合隶属度综合评分法优化杜仲叶发酵工艺。结果表明,杜仲叶的最佳发酵工艺为料液比1∶11、发酵时间42 h、接种量5%。此条件下,杜仲叶发酵液芦丁含量为0.33 mg·g^(-1)、桃叶珊瑚苷含量为0.37 mg·g^(-1)、DPPH·清除率为80.67%、·OH清除率为5.21%。

Using Eucommia ulmoides leaves as raw materials,yeast was used to ferment Eucommia ulmoides leaves.The fermentation process of Eucommia ulmoides leaves was optimized by single factor experiment,orthogonal experiment and comprehensive membership score method.The results showed that the optimum fermentation conditions of Eucommia ulmoides leaves were as follows:the ratio of material to liquid at 1∶11,fermentation time for 42 hours and inoculation amount of 5%.Under these conditions,the content of rutin in the fermentation broth of Eucommia ulmoides leaves was 0.33 mg·g^(-1),the content of aucubin was 0.37 mg·g^(-1),the clearance rate of DPPH·was 80.67%,and the clearance rate of·OH was 5.21%.