虚拟仿真在“生物技术药物学实验”中的应用

Application of Virtual Simulation in“Biotechnical Drug Experiments”

由于许多实验环境无法满足新冠病毒取样和检测的要求,且病原微生物新冠病毒具有强传染性和致病性,学生很难开展感染性实验操作。因此,将虚拟仿真技术与新冠病毒实时荧光定量PCR检测相结合构建了虚拟实验平台,其中涵盖实验导学、临床取样及实时荧光定量PCR检测等主要内容,重点模拟RNA提取、病毒序列获取与分析、引物设计、反转录、实时荧光定量PCR及结果分析等操作。通过虚拟环境,在保障学生安全的基础上,让学生更深入地了解新冠病毒实时荧光定量PCR的检测,大大提高了生物技术药物学实验教学的效率。

Since the experimental environment of the majority of schools cannot meet the requirements of sampling and detection of neocoronavirus in performing real-time fluorescence quantitative PCR,and neocoronavirus is highly infectious and pathogenic during operation,it is difficult for students to carry out the actual experimental operation.Therefore,the virtual simulation technology combined with the real-time fluorescence quantitative PCR detection of the new coronavirus is used to build a virtual experimental platform,which covers the main content of the experimental introduction,clinical sampling and real-time fluorescence quantitative PCR detection,focuses on the simulation of the RNA extraction,viral sequence acquisition and analysis,primer design,reverse transcription,the development of standard curves,real-time fluorescence quantitative PCR and analysis of the results of the operation.Through the virtual environment,students can have a deeper understanding of the detection of real-time fluorescence quantitative PCR of new coronavirus,which greatly improves the efficiency of the experimental teaching of biotechnology pharmacology.