摘要
由于许多实验环境无法满足新冠病毒取样和检测的要求,且病原微生物新冠病毒具有强传染性和致病性,学生很难开展感染性实验操作。因此,将虚拟仿真技术与新冠病毒实时荧光定量PCR检测相结合构建了虚拟实验平台,其中涵盖实验导学、临床取样及实时荧光定量PCR检测等主要内容,重点模拟RNA提取、病毒序列获取与分析、引物设计、反转录、实时荧光定量PCR及结果分析等操作。通过虚拟环境,在保障学生安全的基础上,让学生更深入地了解新冠病毒实时荧光定量PCR的检测,大大提高了生物技术药物学实验教学的效率。
Since the experimental environment of the majority of schools cannot meet the requirements of sampling and detection of neocoronavirus in performing real-time fluorescence quantitative PCR,and neocoronavirus is highly infectious and pathogenic during operation,it is difficult for students to carry out the actual experimental operation.Therefore,the virtual simulation technology combined with the real-time fluorescence quantitative PCR detection of the new coronavirus is used to build a virtual experimental platform,which covers the main content of the experimental introduction,clinical sampling and real-time fluorescence quantitative PCR detection,focuses on the simulation of the RNA extraction,viral sequence acquisition and analysis,primer design,reverse transcription,the development of standard curves,real-time fluorescence quantitative PCR and analysis of the results of the operation.Through the virtual environment,students can have a deeper understanding of the detection of real-time fluorescence quantitative PCR of new coronavirus,which greatly improves the efficiency of the experimental teaching of biotechnology pharmacology.
作者
张倩倩
徐立朋
马志国
郭钟雪
陆小云
王峰
ZHANG Qianqian;XU Lipeng;MA Zhiguo;GUO Zhongxue;LU Xiaoyun;WANG Feng(College of Pharmacy,Jinan University,Guangzhou 510632,China)
出处
《实验室研究与探索》
CAS
北大核心
2024年第8期171-174,193,共5页
Research and Exploration In Laboratory
基金
2023年度第一批暨南大学“金课”建设项目(港澳台侨特色“金课”专项)(10100013)
广州市应用基础研究项目(202201010010)
广东省自然科学基金面上项目(2022A1515012636,2024A1515013257)
2024年度暨南大学教学质量与教学改革工程项目(JD2024003)。
关键词
虚拟仿真技术
生物技术药物学实验
新冠病毒的实时荧光定量PCR检测
virtual simulation technology
biotechnical drug experiments
real-time fluorescence quantitative PCR detection of COVID-19