酸枣仁油的超临界CO_(2)萃取工艺及其抗氧化性研究

Research on the Process Conditions and Antioxidant Properties ofSupercritical CO_(2) Extraction of Sour Jujube Kernel Oil

采用超临界CO_(2)萃取技术对酸枣仁进行油脂萃取。利用单因素实验方法考查了孔径、萃取温度、萃取压力、萃取时间四个因素对酸枣仁出油率的影响,最佳萃取条件下对酸枣仁油脂样品进行GC-MS组分分析及DPPH清除能力测定。结果表明:超临界CO_(2)萃取酸枣仁油的最佳工艺为过筛目数80目,温度为45℃,萃取压力40 MPa,时间30 min,在最佳萃取工艺条件下,出油率为29.40%。GC-MS检测结果表明酸枣仁油含有27种化合物,DPPH的清除率IC_(50)值为29.12 mg/mL。

The supercritical CO_(2) extraction was used to extract oil from sour jujube kernels.The single factor experimental method was used to investigate the effects of particle size,extraction temperature,extraction pressure,and extraction time on the oil yield of sour jujube kernels.Under the optimal extraction conditions,GC-MS component analysis and DPPH scavenging ability determination were performed on the sour jujube kernel oil samples.The results showed that the optimal process for supercritical CO_(2) extraction of sour jujube kernel oil was 80 mesh sieving,45℃temperature,extraction pressure of 40 Mpa,and 30 minutes.Under the optimal extraction process conditions,the oil yield was 29.40%.The GC-MS detection results indicate that sour jujube kernel oil contains 27 compounds,and the IC 50 value of DPPH clearance is 29.12 mg/mL.