硫辛酸对全氟辛酸所致大鼠生殖毒性效应的保护作用

Protective effect of lipoic acid on reproductive toxicity induced by perfluorooctanoic acid in rats

目的探讨成年雄性大鼠经全氟辛酸(PFOS)染毒后,硫辛酸(LA)对大鼠生殖毒性效应的保护作用。方法选取40只成年雄性SD大鼠随机分为正常对照组、LA对照组、PFOS染毒组、低剂量LA组、高剂量LA组。各分组处理如下:正常对照组给予玉米油,LA对照组给予100 mg/kg LA,PFOS染毒组给予10 mg/kg PFOS,低剂量LA组给予10 mg/kg PFOS+50 mg/kg LA,高剂量LA组给予10 mg/kg PFOS+100 mg/kg LA。采用灌胃法连续给药28 d,成模后精确测定大鼠体质量并麻醉处死大鼠。检测大鼠体质量、睾丸质量、附睾质量、睾丸及附睾脏器系数、精子计数。采用苏木精-伊红染色观察大鼠睾丸组织结构的病理学变化。结果PFOS染毒组、低剂量LA组、高剂量LA组大鼠体质量低于正常对照组和LA对照组,差异均有统计学意义(P<0.05);PFOS染毒组、低剂量LA组大鼠附睾质量低于正常对照组和LA对照组,差异均有统计学意义(P<0.05);PFOS染毒组、低剂量LA组、高剂量LA组大鼠睾丸脏器系数高于正常对照组和LA对照组,差异均有统计学意义(P<0.05);PFOS染毒组、高剂量LA组大鼠附睾脏器系数高于正常对照组,差异均有统计学意义(P<0.05);LA对照组大鼠附睾质量、睾丸脏器系数高于正常对照组,大鼠体质量低于正常对照组,差异均有统计学意义(P<0.05)。苏木精-伊红染色结果显示,PFOS染毒处理后,大鼠睾丸生精小管形态结构不完整,间质细胞数量减少,紧密连接结构破坏,各级生精细胞排列紊乱,生精上皮空泡化改变。结论PFOS可导致大鼠生殖系统损伤,LA干预对PFOS所致的生殖毒性效应具有保护作用。

Objective To investigate the protective effect of lipoic acid(LA)on reproductive toxicity in adult male rats exposed to perfluorooctanoic acid(PFOS).Methods A total of 40 adult male SD rats were randomly divided into normal control group,LA control group,PFOS infected group,low dose LA group and high dose LA group.Each group is processed as follows.The normal control group was given corn oil,the LA control group was given 100 mg/kg LA,the PFOS infected group was given 10 mg/kg PFOS,and the low dose LA group was given 10 mg/kg PFOS+50 mg/kg LA.The high dose LA group was given 10 mg/kg PFOS+100 mg/kg LA.After 28 days of continuous administration by intragastric administration,the weight of the rats was accurately determined and the rats were killed by anesthesia.Body mass,testicular mass,epididymal mass,testicular and epididymal organ coefficient and sperm count were measured.The pathological changes of testicular tissue structure were observed by hematoxylin-eosin staining.Results The body mass of rats in PFOS infected,low dose LA and high dose LA groups were lower than those in normal control group and LA control group,and the differences were statistically significant(P<0.05).The epididymal mass of rats in PFOS infected and low dose LA groups were lower than those in normal control group and LA control group,and the differences were statistically significant(P<0.05).The testicular organ coefficient in PFOS infected,low dose LA and high dose LA groups were higher than those in normal control group and LA control group,and the differences were statistically significant(P<0.05).The epididymal organ coefficients in PFOS infected and high dose LA groups were higher than those in normal control group,and the differences were statistically significant(P<0.05).The epididymal mass and testicular organ coefficient of rats in LA control group were higher than those in normal control group,and the body mass of rats were lower than those in normal control group,and the differences were statistically significant(P<0.05).The results of hematoxylin-eosin staining showed that the morphological structure of seminiferous tubules was incomplete,the number of interstitial cells was reduced,the tight junction structure was damaged,the spermatogenic cells at all levels were arranged in disorder,and the spermatogenic epithelium was vacuolated.Conclusion PFOS can cause damage to the reproductive system in rats,and LA intervention can protect the reproductive toxicity induced by PFOS.