单细胞测序在口腔鳞状细胞癌研究中的应用

Application of Single-cell Sequencing in Oral Squamous Cell Carcinoma Research.

口腔鳞状细胞癌是一种常见口腔恶性肿瘤,易转移、复发和产生耐药性。单细胞测序及在此基础上发展而来的空间转录组技术可以获取单个细胞水平的转录组信息,能更加深入地研究细胞种群、功能、变化和通讯。本文从细胞分型、肿瘤微环境、肿瘤侵袭和转移、治疗靶点与预后标志、免疫治疗、肿瘤内微生物等方面对单细胞测序技术在口腔鳞状细胞癌研究中的应用进行归纳总结,并探讨当前研究的不足与未来改进方向。

Oral squamous cell carcinoma(OSCC)is a common malignant oral tumor with a tendency for metastasis,recurrence,and drug resistance.Current research on its mechanisms is insufficient.Single-cell sequencing and the spatial transcriptome technology can obtain transcriptome information at the single-cell level,enabling more in-depth research on cell populations,functions,changes,and communication.This paper provides a comprehensive summary of the application of single-cell sequencing technology in OSCC research areas such as cell typing,tumor microenvironment,tumor invasion and metastasis,therapeutic targets and prognostic markers,immune-targeted therapy,and intratumoral microbiota.It also points out the current deficiencies in research and suggests directions for future improvements.