基于UHPLC-LTQ-Orbitrap-MS/MS技术快速分析六神曲中的化学成分

Rapid Analysis of Chemical Components in Massa Medicata Fermentata Based on UHPLC-LTQOrbitrap-MS/MS Technology

目的建立快速、高效的超高效液相色谱-线性离子阱轨道阱串联质谱联用(UHPLC-LTQ-Orbitrap-MS/MS)方法,系统、全面地对六神曲化学成分进行快速定性分析。方法UHPLC-LTQ-Orbitrap-MS/MS扫描六神曲中化合物的精确分子量,经对照品和多级碎片离子信息,并结合相关文献以及裂解规律,从而对六神曲实现准确的定性分析。结果共鉴定出76种化学成分,包括氨基酸类、核苷类、黄酮类、有机酸类、苯丙素类、萜类、氰苷类、不饱和脂肪酸类等,并对六神曲发酵前后的76种成分的峰面积进行比较,共找出8种峰面积变化显著成分。结论建立了六神曲化学成分的快速定性分析方法,为六神曲的质量评价指标及药效物质基础研究提供了一定的理论依据。

OBJECTIVE To establish a rapid and efficient ultra high performance liquid chromatography-linear ion trap orbital trap tandem mass spectrometry(UHPLC-LTQ-Orbitrap-MS/MS)method for systematic and comprehensive qualitative analysis of chemical components in Massa Medicata Fermentata.METHODS UHPLC-LTQ-Orbitrap-MS/MS was used to scan the precise molecular weight of the compounds in Massa Medicata Fermentata,and accurate qualitative analysis of Massa Medicata Fermentata was achieved through reference substance and multi-stage fragment ion information,combined with relevant literature and fragmentation rules.RESULTS A total of 76 chemical components were identified,including amino acids,nucleosides,flavonoids,organic acids,phenylpropanoids,terpenoids,cyanosides,unsaturated fatty acids,etc.,and the peak area of 76 components before and after fermentation of Massa Medicata Fermentata was compared,and a total of 8 components with significant changes in peak area were found.CONCLUSION A rapid qualitative analysis method for the chemical components of Massa Medicata Fermentata is established.This study provides a theoretical basis for the quality evaluation index and the pharmacodynamic material basis of Massa Medicata Fermentata.