C57BL/6J小鼠与昆明小鼠NIAAA法酒精性肝病造模效果的比较

ComparisoComparison of alcoholic liver disease model in C57BL 6J mice and Kunmingmice using NIAAA methodn of alcoholic liver disease model in C57BL 6J mice and Kunming mice using NIAAA method

为了对比C57BL/6J小鼠和昆明小鼠酒精性肝病模型建造效果及建模完成后续肝脏生化指标变化情况,为酒精性肝病的治疗研究提供基础依据,试验选择雌性C57BL/6J小鼠和昆明小鼠各36只,随机分为对照组(液体饲料+麦芽糊精)、建模16 d组与建模19 d组(液体饲料+酒精,n=12)。利用NIAAA法建造酒精性肝病小鼠模型,并分别于建模第16 d、建模第19 d取其血清及肝组织进行生化指标检测及病理切片观察。结果显示,C57BL/6J小鼠建模16 d组和建模19 d组与昆明小鼠建模16 d组的小鼠血清ALT、AST含量,肝脏MDA、TG含量均分别显著高于其对照组小鼠(P<0.05);而血清ALB含量、肝脏SOD活力显著低于其对照组小鼠(P<0.05)。C57BL/6J建模16 d组和建模19 d组小鼠的肝脏GSH含量和肝脏系数显著高于其对照组小鼠(P<0.05),但昆明小鼠建模19 d组除了肝脏SOD活力显著低于其对照组小鼠(P<0.05),其他指标均与其对照组小鼠无显著差异。病理切片结果表明,与对照组相比,建模16 d组2种小鼠肝细胞排列紊乱,肝细胞索断裂,肝细胞出现炎性浸润和脂肪液滴。C57BL/6J小鼠建模19 d组肝组织仍呈现病变,而昆明小鼠建模19 d组肝细胞已逐渐恢复正常排列。与昆明小鼠相比,C57BL/6J小鼠对于酒精的肝脏氧化应激损伤更严重,酒精性肝病模型更稳定。

To compare the effectiveness of constructing alcoholic liver disease models in C57BL 6J mice and Kunming mice,and analyze the subsequent changes in liver biochemical indicators,this experiment aims to establish a foundational basis for researching the treatment of alcoholic liver disease.A total of 36 female C57BL 6J mice and 36 female Kunming mice were randomly assigned to the control group(liquid chow+maltodextrin),the 16-day modeling group and the 19-day modeling group(liquid chow+alcohol,n=12).The NIAAA method was utilized to induce alcoholic liver disease in the mouse models.Results indicated that in the 16-day and 19-day modeling groups of C57BL 6J mice as well as the 16-day modelling group of Kunming mice,there were significant increases in serum ALT,AST,liver MDA,and TG levels compared to their respective control groups(P<0.05).Conversely,levels of serum ALB and liver SOD were significantly lower in the modeling groups compared to controls(P<0.05).Moreover,liver GSH level and liver coefficient in the 16-day and 19-day modeling groups of C57BL 6J mice were significantly higher than those in the control group(P<0.05).However,in the 19-day modeling group of Kunming mice,except for a lower liver SOD level compared to the control group(P<0.05),there were no other significant differences in indicators.Pathological examination of liver tissue sections revealed disordered arrangement of liver cells,hepatocyte necrosis,inflammatory infiltration and lipid droplets in the 16-day modeling groups of C57BL 6J mice and Kunming mice compared with the control groups.Observation of pathological sections showed that both strains of mice in the 16-day modeling group exhibited disordered arrangement of liver cells,hepatocyte necrosis,inflammatory infiltration and the presence of lipid droplets compared with the control groups.While the liver tissue of C57BL 6J mice in the 19-day modeling group still displayed pathological changes,the liver cells of Kunming mice in the 19-day modelling group had gradually returned to normal arrangement.C57BL 6J mice exhibited more severe alcohol-induced liver oxidative stress damage compared to Kunming mice,resulting in a more stable alcoholic liver model.The experimental results suggest that C57BL 6J mice are more susceptible to alcohol-induced liver oxidative stress damage than Kunming mice,thereby indicating a more stable alcoholic liver model.