石墨炉原子吸收光谱法测定全血铅的方法改进

Improvement of the graphite furnace atomic absorption spectroscopymethod for the determination of blood lead

目的 改进酸脱蛋白-石墨炉原子吸收光谱法测定人体血液中铅的方法。方法 血样标本经酸脱蛋白处理后,用1.00 g/100 mL磷酸二氢铵溶液做基体改进剂,优化石墨炉升温程序,选用Zeeman 2磁场背景校正方式,采用自动进样器配制标准系列,仪器自动扣除试剂空白,在283.3 nm波长下用石墨炉原子吸收分光光度计测定吸光度。结果 本方法在50.0~500.0μg/L范围内线性良好(相关系数r=0.999),不同浓度血铅的相对标准偏差为1.08%~2.08%,不同浓度标准物质检测结果均在允许偏差范围以内,加标回收率为104.9%~106.8%。结论 改进后酸脱蛋白-石墨炉原子吸收光谱法测定血液中的铅,操作简单,干扰小,方法精密度高于国标方法,准确度符合国家规定,适合血液样本中铅含量的测定。

Objective To improve the method of acid-deproteinization graphite furnace atomic absorption spectroscopy for determining lead in human blood.Methods After deproteinizing the blood sample with acid,the 1.00 g/100 mL ammonium dihydrogen phosphate solution was used as a matrix modifier to optimize the graphite furnace heating program.The Zeeman 2 magnetic field background correction method was used,and an automatic sampler was used to prepare the standard series.The instrument automatically deducted the reagent blank and measured the absorbance using a graphite furnace atomic absorption spectrophotometer at the wavelength of 283.3 nm.Results This method had good linearity in the range of 50.0-500.0μg/L(correlation coefficient r=0.999),and the relative standard deviation(RSD)of different concentrations of blood lead was between 1.08%-2.08%.The detection results of standard substances at different concentrations were within the allowable deviation range,and the recovery rate of spiked samples was between 104.9%-106.8%.Conclusions The improved acid-deproteinization graphite furnace atomic absorption spectroscopy method for the determination of lead in blood has simple operation,low interference,and higher precision than the national standard method.Its accuracy meets the national regulations,and it is suitable for the determination of lead content in blood samples.