眼睑基底细胞癌组织中LRIG1、EGFR及GLI1的表达及其相关性研究

Expression and correlation of LRIG1, EGFR, and GLI1 in eyelid basal cell carcinoma

目的:探讨眼睑基底细胞癌组织中亮氨酸重复序列和免疫球蛋白样域蛋白-1(LRIG1)、表皮生长因子受体(EGFR)及神经胶质瘤关联癌基因同源物1(GLI1)的表达情况及其相关性。方法:病例系列报告。纳入蚌埠医科大学第一附属医院2016年1月—2020年11月病理确诊的55例眼睑基底细胞癌患者,其中男26例、女29例,年龄52~89(69.1±9.0)岁。选取55例患者手术切除后保留的癌组织标本,并随机抽取其中22例患者的癌旁正常皮肤组织标本作为对照。观察指标:(1)采用免疫组织化学染色检测并比较LRIG1、EGFR及GLI1在眼睑基底细胞癌组织与癌旁正常皮肤组织中的阳性表达率;(2)比较LRIG1、EGFR及GLI1在不同性别、不同年龄(≥65岁和<65岁)、不同肿块大小(≥2.0 cm和<2.0 cm)患者癌细胞中表达的差异。(3)分析LRIG1、EGFR及GLI1三种蛋白表达水平的相关性。结果:(1)LRIG1在眼睑基底细胞癌组织中阳性表达率为16.4%(9/55),低于在癌旁正常皮肤组织中的阳性表达率72.7%(16/22);而EGFR和GLI1在眼睑基底细胞癌组织中阳性表达率分别为72.7%(40/55)及70.9%(39/55),均高于在癌旁正常皮肤组织中的阳性表达率31.8%(7/22)、27.3%(6/22):差异均有统计学意义(χ^(2)=22.77、11.06、12.32,P值均<0.05)。(2)GLI1、EGFR、LRIG1表达在不同性别、不同年龄及不同肿块大小的患者间差异均无统计学意义(P值均>0.05)。(3)Spearman相关分析表明,55例眼睑基底细胞癌组织中,LRIG1表达分别与EGFR、GLI1表达呈负相关(r=-0.279,P=0.039;r=-0.306,P=0.023);EGFR表达与GLI1表达呈正相关(r=0.499,P<0.001)。结论:EGFR及GLI1在眼睑基底细胞癌组织中均呈高表达,LRIG1呈低表达并分别与两者呈负相关;EGFR及GLI1蛋白可能会导致眼睑基底细胞癌的发生和发展,而LRIG1对癌症的发生发展可能起抑制作用。

Objective This study aimed to investigate the expression of leucine repeat and immunoglobulin-like domain protein 1(LRIG1),epidermal growth factor receptor(EGFR),and glioma-associated oncogene homology 1(GLI1)in eyelid basal cell carcinoma(BCC).Methods This study was case series report.A total of 55 patients with eyelid BCC diagnosed pathologically in the First Affiliated Hospital of Bengbu Medical University from January 2016 to November 2020 were enrolled,including 26 males and 29 females,aged 52-89(69.1±9.0)years.Cancer tissues retained after surgical resection was performed in 55 patients,and the adjacent normal skin tissues of 22 patients were randomly selected as control.The outcome measures were as follows:immunohistochemical staining was performed to detect the positive expression rates of LRIG1,EGFR,and GLI1 in eyelid BCC tissue and adjacent normal skin tissue and their differences;the differences in the expression level of LRIG1,EGFR,and GLI1 in patients with different gender,ages(≥65 years and<65 years),and tumor sizes(≥2.0 cm and<2.0 cm)were compared;the correlation among the three expressions was analyzed.Results The expression rate of LRIG1 in eyelid BCC tissue was 16.4%(9/55),compared with 72.7%(16/22)in adjacent normal skin tissue.The expression level of EGFR and GLI1 was upregulated in eyelid BCC tissues(72.7%[40/55]and 70.9%[39/55],respectively),compared with that in adjacent normal skin tissues(31.8%[7/22];27.3%[6/22]).The differences were statistically significant(χ^(2)=22.77、11.06、12.32;all P values<0.05).In addition,no significant differences in the expression level of GLI1,EGFR,and LRIG1 were found among patients with different gender,ages,and tumor sizes(all P values>0.05).Spearman correlation analysis showed that the positive expression of LRIG1 was negatively correlated with the positive expression of EGFR and GLI1 in eyelid BCC tissues of 55 cases(r=-0.279,P=0.039;r=-0.306,P=0.023).Moreover,EGFR expression was positively correlated with GLI1 expression(r=0.499,P<0.001).Conclusion In eyelid BCC tissues,EGFR and GLI1 are highly expressed,whereas the expression level of LRIG1 is low.The expression of LRIG1 is negatively correlated with EGFR and GLI1,respectively.Therefore,EGFR and GLI1 may lead to the occurrence and development of eyelid BCC,whereas LRIG1 may inhibit the occurrence and development of cancer.