基于SRAP分子标记的郁金香遗传多样性分析

Genetic Diversity Analysis of Tulip Based on SRAP Markers

为探究郁金香(Tulipa gesneriana)种质资源的遗传背景,精准评价和筛选优异种质用于郁金香遗传改良,该研究采用SRAP分子标记分析40个郁金香品种的遗传背景,明晰遗传多样性和亲缘关系。结果表明:在43对SRAP引物中可用于郁金香遗传多样性研究的多态性引物共21对,在40个品种中共扩增出249条清晰稳定的条带,其中多态性条带245条,多态性比率为98.39%。供试的40个品种遗传相似性指数为0.5020~0.8675,遗传多样性参数等位基因数(N*a)、有效等位基因数(N*e)、Nei’s基因多样性指数(H*)、Shannon’s信息指数(I*)和多态性信息含量分别为1.9810、1.5149、0.3042、0.4603和0.3212,供试材料遗传多样性丰富。聚类结果和主坐标分析将40个郁金香品种分为两大类,其中‘Christmas Magical’‘Banja Luka’‘Madame Lefeber’与其他品种亲缘关系较远,在遗传背景上具有一定差异。

In order to explore the genetic background of tulip germplasm resources,accurately evaluate and screen excellent germplasm for genetic improvement of tulips,the genetic background of 40 tulip varieties was analyzed by SRAP molecular markers,and their genetic diversity and relationships were clarified,respectively.The results showed that out of 43 pairs of SRAP primers,21 pairs of polymorphic primers were available for genetic diversity analysis of tulips,and 249 clear and stable bands were amplified in the 40 tulip varieties,of which 245 were polymorphic bands with a polymorphism rate of 98.39%.The genetic similarity index of the 40 tested varieties ranged from 0.5020 to 0.8675,and the genetic diversity parameters including the number of alleles(N*a),number of effective alleles(N*e),Nei’s gene diversity index(H*),Shannon’s information index(I*),and polymorphic information content were 1.9810,1.5149,0.3042,0.4603,and 0.3212,resp-ectively,indicating rich genetic diversity in the tested materials.Cluster analysis and principal coordinate analysis showed that the 40 tulip varieties were classified into two major groups,among which‘Christmas Magical’,‘Banja Luka’,and‘Madame Lefeber’were relatively distant genetic relationships from other varieties and had certain differences in their genetic backgrounds.