继代培养对冬虫夏草菌品质及抗氧化活性的影响

Effect of Subculture on Quality and Antioxidant Activity of Ophiocordyceps sinensis Mycelia

为探究继代培养对冬虫夏草菌品质及抗氧化活性的影响,对冬虫夏草CS17菌株进行继代培养,并测定其在不同继代培养过程中菌丝生物量、核苷类物质组成及含量、抗氧化活性的变化差异。结果表明:冬虫夏草CS17菌株连续培养6代所得菌丝生物量为0.47~0.79 g,在第3代积累量最高,为0.79 g;冬虫夏草CS17菌株连续培养6代所得菌丝体中腺苷、肌苷、鸟苷、胞苷、尿苷总含量为1.005~6.516 mg/g,在第2代样品积累量最高,为6.516 mg/g。不同继代培养代数培养过程中,冬虫夏草菌丝体DPPH自由基、ABTS+自由基清除能力及总还原力均表现为先上升后下降趋势,第2代样品DPPH自由基、ABTS+自由基清除能力及总还原力最强,第3代至第6代逐渐降低。冬虫夏草CS17菌株连续培养6代所得菌丝体中,菌丝生物量在第3代达到最高,5种核苷类总含量在第2代样品中积累量最高,抗氧化活性在第2代样品中表现较好。

To explore the effect of subculture on the quality and antioxidant activity of Ophiocordyceps sinensis mycelia,the changes of O.sinensis CS17 strain in mycelial biomass,nucleosides accumulation,and antioxidant activity were investigated during subculture processes.The results showed that the mycelial biomass obtained by continuous culture was 0.47-0.79 g,which reached the highest in the third⁃generation samples(0.79 g).The total contents of five nucleosides(adenosine,inosine,guanosine,cytidine,and uridine)of O.sinensis my⁃celia obtained by continuous culture were1.005-6.516 mg/g.Moreover,the highest accumulation of total nucleosides was observed in the second⁃generation samples(6.516 mg/g).In different times of subculture,the DPPH and ABTS+free radicals scavenging activities and total reducing power of O.sinensis CS17 mycelia tended to go up and down,which were the strongest in the second generation and gradually decreased from the third to the sixth.Among the mycelia of O.sinensis CS17 continuously cultured for six generations,the third generation had the largest mycelial biomass.The total contents of nucleosides and antioxidant activities of my⁃celia were highest in the second⁃generation samples.