栽培基质与EM菌剂对卷叶湿地藓生长的影响研究

Influences of Culture Substrate and EM Microbial Agent on the Growth of Hyophila Involuta

为探索卷叶湿地藓的快速扩繁方法,以卷叶湿地藓为研究对象,采用二因素裂区试验设计,设置4种栽培基质和3种EM菌剂浓度,综合分析和评价栽培基质和EM菌剂浓度对卷叶湿地藓生长及生理指标的影响。结果表明:卷叶湿地藓的发枝数、盖度、叶绿素和可溶性糖含量表现为随EM菌剂浓度增高而增加,丙二醛(MDA)随EM菌剂浓度增高而降低。高浓度处理A3B2的发枝数是低浓度处理A3B1的1.16倍,是对照处理A3CK的1.88倍,A3B2处理盖度、叶绿素和可溶性糖含量较对照处理A3CK相比分别增加了57.34%、67.95%和16.94%,较低浓度处理A3B1分别增加了17.30%、29.41%和3.32%,MDA含量较对照A3CK和低浓度处理A3B1分别降低了70.54%和31.56%。园林废弃物堆肥基质处理的发枝数、盖度、叶绿素和可溶性糖含量均略高于草炭基质,A3B2的发枝数、盖度、叶绿素和可溶性糖含量较A4B2处理增加了3.33%、3.83%、4.68%和12.85%,MDA含量较A4B2处理降低了17.47%。隶属函数综合评价结果显示A3B2综合评分最高。综上,栽培基质和EM菌剂对卷叶湿地藓的生长有一定的影响,较适宜卷叶湿地藓的栽培基质和EM菌剂组合为工程弃土40%+园林废弃物堆肥60%配施EM菌剂600倍稀释液。

To explore the rapid propagation method of Hyophila involuta,Hyophila involuta was taken as the research object,and a two factor split zone experimental design was adopted.Four cultivation substrates and three EM microbial agent concentrations were set up to comprehensively analyze and evaluate the effects of cultivation substrate and EM bacterial concentration on the growth and physiological indicators of Hyophila involuta.The results showed:the number of branches,coverage,chlorophyll and soluble sugar content increased with the increase of EM microbial agent concentration.MDA content decreased with increasing EM microbial agent concentration.The number of branches in high concentration treatment A3B2 was 1.16 times higher than that in low concentration treatment A3B1,and 1.88 times higher than that in control treatment A3CK.The coverage,chlorophyll,and soluble sugar content in A3B2 treatment increased by 57.34%,67.95%,and 16.94%respectively compared to control treatment A3CK,while the lower concentration treatment A3B1 increased by 17.30%,29.41%,and 3.32%,respectively.The MDA content decreased by 70.54%and 31.56%compared to control A3CK and low concentration treatment A3B1,respectively.The number of branches,coverage,chlorophyll,and soluble sugar content in the treatment of adding garden waste compost were slightly higher than those in the peat substrate.The number of branches,coverage,chlorophyll,and soluble sugar content in A3B2 increased by 3.33%,3.83%,4.68%and 12.85%compared to A4B2;MDA content decreased by 17.47%compared to A4B2 treatment.The membership function results showed the A3B2 treatment had the highest comprehensive score.In conclusion,the cultivation substrate and EM have a certain impact on the growth of Hyophila involuta.The suitable combination of cultivation substrate and EM concentration for Hyophila involuta is 40%engineering spoil+60%garden waste compost,cooperate with spraying EM with 600x diluent.