分子标记辅助选择W_(x)^(mp)基因改良晚粳品种宁84稻米品质

Molecular Marker-Assisted Selection of W_(x)^(mp)Gene for Improving Rice Quality of Late Japonica Rice Ning 84

为改良晚粳水稻品种宁84的稻米品质,培育低直链淀粉含量的软米粳稻新品系,利用携带软米基因W_(x)^(mp)的水稻品种南粳9108为供体亲本,以晚粳品种宁84为受体亲本,利用分子标记辅助选择将W_(x)^(mp)基因回交导入到宁84中,创制了宁84(W_(x)^(mp))改良系。结果显示:宁84(W_(x)^(mp))改良系的农艺性状与宁84差异不显著,其背景回复率达到98.7%;与宁84相比,其直链淀粉含量由17%降低到10%左右,胶稠度由70 mm增加到最高80 mm;改良系的垩白度和垩白粒率显著增高,透明度显著降低,碱消值保持不变,整精米率和精米率显著高于对照,食味品质明显提升。宁84(W_(x)^(mp))改良系的成功培育,为今后粳稻软米新品系的选育及推广打下了良好基础。

In order to improve the rice quality of the late Japonica variety Ning 84 and to cultivate a new japonica rice line with low amylose content,we used the rice variety Nanjing 9108,which carries the softer rice gene W_(x)^(mp),as the donor pa-rent,and the late Japonica variety Ning 84 as the recipient parent,and backcross-transferred the W_(x)^(mp)gene to Ning 84 by using a functional molecular marker,and created an improved line of Ning 84(W_(x)^(mp)).The agronomic traits of Ning 84(W_(x)^(mp))improved line did not differ significantly from those of Ning 84,and the background recovery rate of which reached 98.7%.Compared with Ning 84,the amylose content of Ning 84(W_(x)^(mp))was reduced from 17%to 10%,the gel consisten-cy increased from 70 mm to 80 mm,the chalkiness and chalky white grain rate of the improved line increased significantly,the transparency decreased significantly,the alkaline elimination value remained unchanged,the whole and polished rice rate was significantly higher than that of the control.The taste quality was significantly improved.The successful breeding of Ning 84(W_(x)^(mp))improved lines has laid a good foundation for the selection and promotion of japonica softer rice in the future.