人参皂苷Rg_(3)对氧化应激诱导神经细胞损伤的保护作用

Protective Effect of Ginsenosides Rg_(3)against Nerve Cell Injury Induced by Oxidative Stress

该文探讨了人参皂苷Rg_(3)对氧化应激诱导PC12细胞损伤的保护作用。通过细胞抗氧化活性(CAA)实验检测Rg_(3)对氧自由基诱导细胞氧化的抑制作用,并建立H_(2)O_(2)诱导的PC12细胞氧化应激模型,CCK-8法检测Rg_(3)对细胞的保护和毒性作用,运用Western blot检测Rg_(3)对炎症和凋亡相关信号通路中p65磷酸化蛋白和CREB磷酸化蛋白的影响。结果发现Rg_(3)对氧自由基的抗氧化能力约是Trolox的7.3倍。200μmol/L H_(2)O_(2)诱导PC12氧化应激时细胞活力下降至60.11%,p-p65蛋白相对量为1.30,p-CREB蛋白相对量为0.80。10、20和40μmol/L Rg_(3)保护组的细胞活力分别提高至74.00%、76.04%、80.36%,呈浓度依赖式提高;细胞p-p65蛋白相对量分别降低至1.18、1.03、0.82;细胞p-CREB蛋白相对量分别提高至1.31、1.75、2.30。结果表明人参皂苷Rg_(3)可能通过清除氧自由基,参与NF-κB/CREB通路调控,减轻PC12细胞炎症和凋亡,抑制氧化应激诱导的神经细胞损伤,为人参的神经营养特性研究及相关功能食品开发提供理论依据。

To determine protective effect of ginsenoside Rg_(3)on oxidative stress-induced injury in PC12 cells,the inhibitory effect of Rg_(3)on oxygen free radicals induced cell oxidation was detected in a cellular antioxidant activity experiment.To this end,an oxidative stress model of PC12 cells induced by H_(2)O_(2)was established,whose cell viability was detected using the CCK-8 method.The effects of Rg_(3)on the protein expression of p65 and CREB,related to inflammatory and apoptosis,were detected by western blotting.The results showed that the antioxidant ability of Rg_(3)in response to oxygen free radicals was approximately 7.3-fold that of Trolox.Cell viability was decreased by 60.11%after treatment with 200μmol/L H_(2)O_(2).Meanwhile,the relative expression of p-p65 protein was increased to 1.30,and p-CREB protein was decreased to 0.80.The cell viabilities of protective groups pretreated with different concentrations of Rg_(3)(10,20,and 40μmol/L)were found to increase by 74.00%,76.04%,and 80.36%,respectively.The relative expressions of p-p65 protein decreased to 1.18,1.03,and 0.82,respectively.Meanwhile,the relative expression of p-CREB protein increased to 1.31,1.75 and 2.30,respectively.These results suggest that ginsenoside Rg_(3)may protect PC12 cells from oxidative damage by scavenging oxygen free radicals.The underlying mechanisms may be related to the NF-κB/CREB pathway,which reduces inflammation and apoptosis in PC12 cells,thereby inhibiting the oxidative stress-induced nerve cell damage.These findings provide a theoretical basis for the study of the neurotrophic characteristics of ginseng and the development of related functional foods.