响应面法优化酸提取荷叶中生物碱的工艺研究

The Response Surface Method Optimizes the Process of Extracting Alkaloids from Lotus Leaves

以荷叶为原材料,在酸浸法和单因素实验法的基础上,结合超声波辅助提取和BoxBehnken响应面分析作图的方法优化荷叶生物碱盐酸提取工艺,以荷叶碱得率为指标,考察超声时间(min)、超声功率(W)、超声温度(℃)、盐酸质量分数(%)对荷叶碱得率的影响,得到荷叶碱提取的最佳提取条件和最大得率.通过方差分析得出,得率变化受到超声时间和盐酸质量分数的影响最大,而超声温度最小,优化后最优提取工艺条件:超声时间为37 min、超声功率为530 W、超声温度为48℃、盐酸质量分数为0.56%时,荷叶碱的最佳提取率为(1.69±0.05)mg/g.

Based on the acid leaching method and the single-factor experimental method,this experiment optimizes the lotus leaf alkaloid extraction process by combining the method of ultrasonic auxiliary extraction and Box-Behnken response surface analysis,and uses the lotus leaf alkali yield as an indicator to investigate the effect of ultrasonic time(min),ultrasonic power(W),ultrasonic temperature(C),hydrochloric acid mass fraction(%)on the yield of lotus leaf alkali,and obtains the optimal extraction condition and maximum yield of lotus leaf base.The test shows that:through Variance analysis method,the resulting rate change is the most affected by ultrasonic time and hydrochloric acid mass fraction,while the ultrasonic temperature is the smallest,the optimal extraction process conditions after optimization:when the ultrasonic time is 37 min,the ultrasonic power is 530 W,the ultrasonic temperature is 48 degrees C,and the hydrochloric acid mass fraction is 0.56%,the optimal extraction rate of lotus base is(1.69±0.05)mg/g.