高级别浆液性卵巢癌类器官模型的建立及鉴定

Establishment and identification of organoid model of high-grade serous ovarian cancer

目的目的建立并鉴定高级别浆液性卵巢癌类器官模型。方法方法选取行卵巢癌细胞减灭术的10例高级别浆液性卵巢癌患者的卵巢癌组织,将卵巢癌组织消化成单细胞,在基质胶中培养7天,构建高级别浆液性卵巢癌类器官模型。比较类器官与原发肿瘤的形态学特征、免疫组化特征和苏木精-伊红(HE)染色结果。结果结果10例患者的高级别浆液性卵巢癌类器官模型均成功构建。光学显微镜显示类器官数量可观,形态呈类圆形3D立体结构。HE染色显示,在高倍镜下,类器官细胞核异型性明显,局部区域形成微乳头状结构。免疫组化显示黏蛋白16(MUC16)强阳性,p53弥漫性强阳性,类器官与原发肿瘤的免疫组化染色情况一致。结论结论体外成功构建的高级别浆液性卵巢癌类器官模型具有明显的上皮性特征,与原发肿瘤高度相似,可用于卵巢癌的药物筛选和基础研究建模。

Objective To establish and identify the organoid model of high-grade serous ovarian cancer.Method Ovarian cancer tissues of 10 patients with high-grade serous ovarian cancer who underwent ovarian reduction were select-ed.The ovarian cancer tissues were digested into monoplast and cultured in matrix gel for 7 days to construct high-grade serous ovarian cancer organoid model.The morphologic,immunohistochemical characteristics and hematoxylin-eosin(HE)staining results of organoids and primary tumors were compared.Result Organoid models of high-grade serous ovarian cancer were successfully constructed in 10 patients.The optical microscope showed that the organoids were abun-dant and the shapes were like circular 3D stereoscopic structure.HE staining showed that the nuclei of organoids were het-eromorphic at high power lens,and micropapillary structures were formed in local areas.Immunohistochemistry showed strong positive expression of mucin 16(MUC16),strong diffuse positive expression of p53,and immunohistochemical staining of organoids and primary tumors were consistent.Conclusion The successfully constructed high-grade serous ovarian cancer organoid model has obvious epithelial characteristics and is highly similar to the primary tumors,which can be used for drug screening and basic research modeling of ovarian cancer.