基于GC−MS/O和OAV技术分析雪茄烟叶关键香气物质

Analysis of key aroma components of cigar tobacco leaves by GC-MS/O and OAV method

为分析雪茄烟叶的关键香气物质,采用同时蒸馏萃取(SDE)和固相微萃取(SPME)法对香气物质进行提取,利用气相色谱–质谱/嗅闻联用技术(GC−MS/O)和香气活力值(OAV)技术对雪茄烟叶香气物质进行确定,并通过香气重组实验进行验证.结果表明:①两种提取方法共得到75种香气物质,包括醇类、醛类、酯类、酸类、烃类、杂环类和其他类;②SDE法所得雪茄烟叶提取物主要体现花香和果香香韵,SPME法所得雪茄烟叶提取物主要体现果香香韵;③结合GC−MS/O和OAV技术能够有效确定雪茄烟叶的关键香气物质.基于上述分析结果的香气重组物进行卷烟加香实验能够彰显雪茄特征风味,显著提高卷烟的感官品质.

To analyze the key aroma compounds in cigar tobacco leaves,simultaneous distillation extraction(SDE)and solid-phase microextraction(SPME)methods were used to extract the aroma compounds.Gas chromatography-mass spectrometry/olfactory coupling technology(GC-MS/O)and aroma vitality value(OAV)technology were used to determine the aroma quality of cigar tobacco leaves,and were validated through aroma recombination experiments.The results showed that:①Two extraction methods obtained a total of 75 aroma substances,including alcohols,aldehydes,esters,acids,hydrocarbons,heterocycles,and other types.②The cigar tobacco extract obtained by SDE method mainly reflected floral and fruity aroma,while the cigar tobacco extract obtained by SPME method mainly exhibited fruity aroma.③The combination of GC-MS/O and OAV techniques can effectively determine the key aroma compounds in cigar tobacco leaves.Based on the above analysis results,conducting cigarette flavoring experiments with aroma recombinants can demonstrate the characteristic flavor of cigars and significantly improve the sensory quality of cigarettes.