小球藻-好氧污泥耦合颗粒的培养及处理特性研究

Cultivation and treatment characteristics of Chlorella-aerobic sludge coupled system

在实验室条件下培养出好氧颗粒污泥作为生物载体,接种小球藻形成菌藻共生颗粒污泥(Algal-bacterial granular sludge,ABGS),探究不同光照强度对ABGS系统去除污染物效果的影响,重点考察了5 klx光照强度下小球藻对ABGS系统处理能力的增强作用。结果表明,在0~100 klx光照强度范围内,ABGS对COD、TN和TP的去除率分别为87.01%~95.32%、53.14%~89.37%和32.49%~99.40%,同步硝化反硝化率(SND)为7.70%~83.66%;而单一小球藻系统对TP和TN的去除率仅为20%~30%,对COD去除效果不明显。在一定光照强度范围内,小球藻可以提高水中DO,并通过促进ABGS系统中细菌的代谢速率来促进污染物的降解。随着光照强度的增强,ABGS系统对COD和TP的去除率上升,对TN的去除率下降,SND下降。过低或过高的光照强度均会抑制小球藻的光合作用甚至使其死亡,导致ABGS破裂解体,系统崩溃,污染物去除效果变差。高通量分析表明,藻类的加入进一步扩大了与氮磷代谢相关的菌种相对丰度,增强了系统的脱氮除磷效果。

Aerobic granular sludge was cultivated as a biological carrier under laboratory conditions,and Chlorella was inoculated to form algal-bacterial granular sludge(ABGS).The effects of different light intensities on pollutant removal efficiency of ABGS system were explored,and the enhancement of Chlorella on the treatment capacity of ABGS system under 5 klx light intensity was emphatically investigated.The results showed that the removal rates of COD,TN and TP by ABGS were 87.01%-95.32%,53.14%-89.37%and 32.49%-99.40%,respectively,with the SND of 7.70%-83.66%.However,the removal rates of TP and TN in a single Chlorella system were only 20%-30%,while the removal effect of COD was not obvious.Chlorella could increase the concentration of dissolved oxygen in water within a certain light intensity range,and facilitate the degradation of pollutants by promoting the metabolic rate of bacteria in ABGS system.With the increase of light intensity,the removal rates of COD and TP in ABGS system increased,while the removal rates of TN and SND decreased.Too low or too high light intensity would inhibit the photosynthesis and even kill Chlorella,leading to the disintegration of ABGS,system collapse and poor pollutant removal effect.High throughput analysis showed that the addition of algae further expanded the relative abundance of bacteria related to nitrogen and phosphorus me⁃tabolism,and enhanced the nitrogen and phosphorus removal effect of the system.