PSMD6在肝细胞癌中的异常高表达及临床价值

Abnormal High Expression and its Clinical Value of PSMD6 in Hepatocellular Carcinoma

目的评估PSMD6在肝细胞癌(HCC)患者组织中的表达情况,并探究其潜在临床价值。方法基于Gene Expression Omnibus(GEO)、Metabric、TCGA-GTEx、ArrayExpress、Sequence Read Archive(SRA)数据库,综合分析全球多中心、多平台的PSMD6基因表达谱,探究PSMD6的mRNA在HCC中的过表达趋势。同时,使用The Human Protein Atlas组织芯片库展示PSMD6在HCC组织中蛋白表达情况。绘制KM曲线,分析PSMD6在HCC中的预后预测价值。结果PSMD6蛋白表达的阳性染色信号定位于HCC细胞的细胞浆和核膜中,表达强度中等,染色度中等。HCC中PSMD6高表达(P<0.01),结果未发生偏倚。SROC的AUC为0.75(0.71,0.79),敏感性为0.64,特异性为0.74,提示PSMD6具有中度鉴别HCC的能力。生存分析显示,PSMD6高表达的患者面临着更高的生存风险(HR=1.4,P=0.043)。结论PSMD6在HCC中异常高表达,可作为HCC筛查和治疗潜在靶点。

Objective To evaluate the expression of PSMD6 in patients with hepatocellular carcinoma(HCC),and explore its potential clinical value.Methods Based on Gene Expression Omnibus(GEO),Metabric,TCGA-GTEx,ArrayExpress,Sequence Read Archive(SRA)databases,the global multi-center and multi-platform PSMD6 gene expression profiles were comprehensively analyzed to explore the overexpression trend of PSMD6 mRNA in HCC.At the same time,the Human Protein Atlas tissue microarray library was used to show the protein expression of PSMD6 in HCC tissues.KM curve was drawn to analyze the prognostic value of PSMD6 in HCC.Results The positive staining signal of PSMD6 protein expression was located in the cytoplasm and nuclear membrane of HCC cells,with moderate expression intensity and moderate staining degree.PSMD6 was highly expressed in HCC(P<0.01),and the results were not biased.The AUC of SROC was 0.75(0.71,0.79),the sensitivity was 0.64,and the specificity was 0.74,suggesting that PSMD6 had a moderate ability to identify HCC.Survival analysis showed that patients with high expression of PSMD6 with a higher survival risk(HR=1.4,P=0.043).Conclusion PSMD6 is abnormally highly expressed in HCC,which can be used as a potential target for HCC screening and treatment.